Details Matter: Designing Skin Microbiome Studies.
Details Matter: Designing Skin Microbiome Studies.
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DOI：
10.1016/j.jid.2016.03.004
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发表时间：
2016-05
期刊：
影响因子：
--
通讯作者：
Kong HH
中科院分区：
文献类型：
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作者：
Kong HH
基因组测序在研究微生物方面的应用已经扩大，但也提出了关于研究皮肤微生物组的最佳方法的问题。在本期中，Meisel等人证明，虽然全基因组霰弹枪元基因组测序与预期的微生物图谱最相似，但16S rRNA基因的V1-V3测序在确定突出皮肤细菌的属和种水平分类方面比V4测序具有更高的准确性。
The use of genomic sequencing to investigate microbes has expanded, yet has also raised questions regarding optimal approaches to studying the skin microbiome. In this issue, Meisel et al. demonstrate that while whole genome shotgun metagenomic sequencing was most similar to the expected microbial profiles, V1–V3 sequencing of the 16S rRNA gene had greater accuracy than V4 sequencing in determining genus and species level classification of prominent skin bacteria.