Distinct claudin expression profile in histologic subtypes of lung cancer
Distinct claudin expression profile in histologic subtypes of lung cancer
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DOI：
10.1016/j.lungcan.2007.02.018
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发表时间：
2007-08-01
期刊：
影响因子：
5.3
通讯作者：
Kiss, Andras
中科院分区：
文献类型：
--
作者：
Moldvay, Judit;Jaeckel, Marta;Kiss, Andras
目的：claudin是紧密连接的主要成分。对于它们在正常支气管上皮和肺癌中的表达和定位知之甚少。患者和方法：研究了104例肺癌组织块，其中腺癌（ADC） 46例，鳞状细胞癌（SCC） 30例，小细胞肺癌（SCLC） 15例，典型类癌8例，非典型类癌5例。所有载玻片均含有正常支气管黏膜。使用抗claudin -1、-2、-3、-4和-7蛋白的抗体进行免疫组化，并进行半定量估计。对22例具有免疫组织化学代表性的肿瘤样本进行RT-PCR分析。结果：正常支气管上皮细胞表达所有的claudin蛋白。相比之下，SCCs和类癌在claudin -1、-3和-4的表达方面存在显著差异（分别为p < 0.0001、p < 0.0001和p < 0.0004），而adc和SCCs在claudin -3、-4和-7的表达方面存在显著差异（分别为p < 0.0001、p < 0.0001和p < 0.0053）。然而，adc与sclc的比较显示，只有claudin-2的表达差异有统计学意义（p < 0.0002）。adc与类癌比较，claudin -1、-3、-4的表达差异有统计学意义（p < 0.0006、p < 0.0001、p < 0.0001）。SCCs和SCCs中claudin-2、-3和-4的表达存在差异（p < 0.0009、p < 0.0001和p < 0.0019），而SCCs和类癌中claudin- 1和-4的表达存在差异（p < 0.0076和p < 0.0045）。RT-PCR分析显示，某些claudin的mRNA和蛋白表达发生了平行变化。结论：在非小细胞肺癌组中观察到不同的claudin表达谱，进一步，scclc和类癌之间的显著差异可能具有鉴别诊断的意义，某些claudin的过表达可能具有治疗意义。2007爱思唯尔爱尔兰有限公司版权所有。
Purpose: Claudins are the main constituents of tight junctions. Little is known about their expression and localization in the normal bronchial epithelium and in lung cancer.Patients and methods: One hundred four lung cancer tissue blocks were studied including 46 adenocarcinomas (ADC), 30 squamous cell carcinomas (SCC), 15 small cell lung cancers (SCLC), 8 typical and 5 atypical carcinoids. All slides contained normal bronchial mucosa as well. Immunohistochemistry using antibodies against claudins-1, -2, -3, -4, and -7 proteins, as well as semi-quantitative estimation were performed. RT-PCR analysis was also carried out in 22 immunohistochemically representative tumor samples.Results: Normal bronchial epithelial cells expressed all the examined claudin proteins. When compared, SCLCs and carcinoids showed striking differences in regard to claudins-1, -3, and -4 expressions (p < 0.0001, p < 0.0001, and p < 0.0004, respectively), whereas ADCs and SCCs revealed significant differences in claudins-3, -4, and -7 expressions (p < 0.0001, p < 0.0001, and p < 0.0053, respectively). However, comparison of ADCs with SCLCs revealed significant difference only in claudin-2 expression (p < 0.0002). The comparison of ADCs and carcinoids resulted in significant differences regarding claudins-1, -3, and -4 expressions (p < 0.0006, p < 0.0001, and p < 0.0001, respectively). SCCs and SCLCs varied in respect to claudin-2, -3, and -4 expressions (p < 0.0009, p < 0.0001, and p < 0.0019, respectively), whereas SCCs and carcinoids showed different claudins-1 and -4 expressions (p < 0.0076 and p < 0.0045, respectively). RT-PCR analysis revealed parallel changes in the mRNA and protein expression of certain claudins.Conclusions: The observed distinct claudin expression profile within the non-small cell lung cancer group, further, the marked differences between SCLCs and carcinoids may have differential diagnostic impact, and the overexpression of certain claudins might have therapeutic implications. 2007 Elsevier Ireland Ltd. All rights reserved.