Angio-associated migratory cell protein (AAMP) interacts with cell division cycle 42 (CDC42) and enhances migration and invasion in human non-small cell lung cancer cells.
Angio-associated migratory cell protein (AAMP) interacts with cell division cycle 42 (CDC42) and enhances migration and invasion in human non-small cell lung cancer cells.
复制标题
血管相关迁移细胞蛋白 (AAMP) 与细胞分裂周期 42 (CDC42) 相互作用，增强人非小细胞肺癌细胞的迁移和侵袭。
DOI：
10.1016/j.canlet.2020.11.050
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发表时间：
2021
期刊：
影响因子：
9.7
通讯作者：
Su Ling
中科院分区：
文献类型：
--
作者：
Yao Shun;Shi Feifei;Mu Ning;Li Xiaopeng;Ma Guilin;Wang Yingying;Sun Xiaoyang;Liu Xiangguo;Su Ling
血管相关迁移细胞蛋白(AAMP)被认为是一种促肿瘤蛋白，参与血管生成、增殖、黏附等生物学活性。虽然已知AAMP通过调节ras同源家族成员A(RHOA)的活性促进乳腺癌细胞和平滑肌细胞的运动，但AAMP在非小细胞肺癌(NSCLC)细胞转移中的作用仍不清楚。在本研究中，AAMP在非小细胞肺癌中表达上调，并被发现促进了NSCLC细胞的迁移和侵袭。进一步的实验证明，AAMP与细胞分裂周期42(CDC42)相互作用并促进其激活，导致细胞突起的形成。随后，我们发现AAMP通过破坏Rho GTP酶激活蛋白1(ARHGAP1)与CDC42的结合而增强了CDC42的活性。综上所述，我们揭示和阐明了AAMP在NSCLC细胞迁移和侵袭中的关键作用，并为肺癌的治疗提供了一个新的潜在靶点。
Angio-associated migratory cell protein (AAMP) is considered a pro-tumor protein, which contributes to angiogenesis, proliferation, adhesion, and other biological activities. Although AAMP is known to facilitate the motility of breast cancer cells and smooth muscle cells by regulating ras homolog family member A (RHOA) activity, the function of AAMP in the metastasis of non-small cell lung cancer (NSCLC) cells still remains unknown. In the present study, AAMP was upregulated in non-small cell lung carcinoma, and was found to promote migration and invasion in NSCLC cells. Further experiments demonstrated that AAMP interacted with cell division cycle 42 (CDC42) and promoted its activation, resulting in the formation of cellular protrusions. Subsequently, we found that AAMP enhanced CDC42 activation by impairing the combination of rho GTPase activating protein 1 (ARHGAP1) and CDC42. Taken together, we revealed and elucidated the critical role of AAMP in the migration and invasion of NSCLC cells and presented a new potential target for lung cancer therapy.