Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2
Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2
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DOI：
10.1006/jmbi.1999.3280
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发表时间：
1999-12-03
影响因子：
5.6
通讯作者：
Subirana, JA
中科院分区：
文献类型：
--
作者：
Abrescia, NGA;Malinina, L;Subirana, JA
We present the structure of the decanucleotide d(CGTATATACG) determined by single crystal X-ray diffraction at 1.58 A resolution. A netropsin drug is found in the minor groove with guanine stacked on a pyrrole ring of the drug, a feature described here for the first time. The stacked guanine is an extra-helical base coming from the end of a neighbour, oligonucleotide. This observation may open the way to the development of minor groove binding drugs with a higher sequence selectivity. The oligonucleotide is in the B-conformation, but the terminal base-pairs are disrupted the cytosine residues are disordered while the guanine residues penetrate into the minor groove of neighbouring duplexes. Four hydrated Ni ions with octahedral co-ordination are found associated with the N-7 atoms of each guanine. The high affinity of these ions with guanine suggests that they may be used as probes for specific guanine residues. (C) 1999 Academic Press.
