A circular cross-section PDMS microfluidics system for replication of cardiovascular flow conditions
A circular cross-section PDMS microfluidics system for replication of cardiovascular flow conditions
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DOI：
10.1016/j.biomaterials.2010.01.082
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Kumacheva, Eugenia
中科院分区：
文献类型：
--
作者：
Fiddes, Lindsey K.;Raz, Neta;Kumacheva, Eugenia
自从软光刻技术问世以来，使用软光刻方法可以轻松且经济高效地制造用于心血管研究的微流体装置。这种方法的缺点是制造具有矩形横截面的微通道，它不能复制血管的圆形横截面。本文提出了一种使用软光刻在聚二甲基硅氧烷 (PDMS) 中制造具有圆形横截面的微通道的新颖、简单的方法。该方法利用当液体有机硅低聚物同轴引入具有矩形横截面的微通道时，液体有机硅低聚物在气流周围聚合。我们证明了（i）控制微通道圆形横截面直径的能力。 40-100微米； (ii)具有收缩的微通道的制造，以及(iii)在微通道的内表面上生长内皮细胞的能力。 (C) 2010 Elsevier Ltd. 保留所有权利。
Since the inception of soft lithography, microfluidic devices for cardiovascular research have been fabricated easily and cost-effectively using the soft lithography method. The drawback of this method was the fabrication of microchannels with rectangular cross-sections, which did not replicate the circular cross-sections of blood vessels. This article presents a novel, straightforward approach for the fabrication of microchannels with circular cross-sections in poly(dimethylsiloxane) (PDMS), using soft lithography. The method exploits the polymerization of the liquid silicone oligomer around a gas stream when both of them are coaxially introduced in the microchannel with a rectangular cross-section. We demonstrate (i) the ability to control the diameter of circular cross-sections of microchannels from ca. 40-100 mu m; (ii) the fabrication of microchannels with constrictions, and (iii) the capability to grow endothelial cells on the inner surface of the microchannels. (C) 2010 Elsevier Ltd. All rights reserved.