Fine mapping of a major QTL controlling panicle number in rice
Fine mapping of a major QTL controlling panicle number in rice
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控制水稻穗数的主要QTL的精细定位
DOI：
10.1007/s11032-010-9420-6
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发表时间：
2011-02-01
影响因子：
3.1
通讯作者：
Liang, Guohua
中科院分区：
文献类型：
--
作者：
Zhu, Jinyan;Zhou, Yong;Liang, Guohua
水稻单株穗数是粮食产量的组成部分，直接影响水稻产量。控制穗数基因的鉴定将在水稻高产育种中发挥重要作用。 C3074是光鲁爱4号遗传背景中含有日本晴基因渗入片段的染色体片段替换系，其圆锥花序明显较少。对C3074与广鲁爱4号杂交F2代和F3代的数量性状位点（QTL）分析表明，位于1号染色体长臂上的主要QTL qPN1是造成这种表型变异的原因。qPN1还影响株高、穗长、穗粒数和单株产量。遗传分析表明qPN1作为单一孟德尔因子，来自日本晴的等位基因减少了单株的穗数。对 F2 和 F3 代总共 1,429 个隐性个体进行精细定位，根据可用的序列注释数据库将基因定位到包含 6 个注释基因的 34.4 kb 区域。这些信息将有助于今后候选基因的鉴定和分离。我们为 qPN1 开发的紧密连锁标记将成为水稻改良计划中该基因标记辅助选择的有用工具。
Rice panicle number per plant is a grain yield component that directly influences rice yield. The identification of the genes controlling panicle number will play a vital role in high-yield rice breeding. C3074, a chromosome segment substitution line containing an introgression segment of Nipponbare in the genetic background of Guangluai 4, had significantly fewer panicles. Quantitative trait locus (QTL) analysis in F2 and F3 generations derived from a cross between C3074 and Guangluai 4 revealed thatqPN1, a major QTL locating on the long arm of chromosome 1, was responsible for this phenotypic variation.qPN1also affected plant height, panicle length, grain number per panicle and grain yield per plant. Genetic analysis indicated thatqPN1acted as a single Mendelian factor, and that the allele from Nipponbare decreased the panicle number per plant. Fine mapping was carried out with a total of 1,429 recessive individuals from F2 and F3 generations, localizing the gene to a 34.4-kb region containing six annotated genes according to available sequence annotation databases. This information will be helpful for future identification and isolation of the candidate gene. The tightly linked markers that we have developed forqPN1will be a useful tool in the marker-assisted selection of this gene in rice improvement programs.