Co-infection with Wolbachia and Cardinium may promote the synthesis of fat and free amino acids in a small spider, Hylyphantes graminicola
Co-infection with Wolbachia and Cardinium may promote the synthesis of fat and free amino acids in a small spider, Hylyphantes graminicola
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DOI：
10.1016/j.jip.2019.107307
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发表时间：
2020-01-01
影响因子：
3.4
通讯作者：
Peng, Yu
中科院分区：
文献类型：
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作者：
Li, Chunfen;He, Meng;Peng, Yu
内共生细菌与其宿主之间的联系在自然界中广泛存在，并已得到广泛证实；然而，只有少数研究研究了兼性共生体如何影响宿主的营养和代谢。为了深入了解兼性共生体与宿主营养和代谢活性之间的关系，我们检测了一种小蜘蛛种Hylyphantes graminicola的内共生感染，并建立了两种感染菌株，即W-C+ （Wolbachia阴性，Cardinium阳性）和W+C+ （Wolbachia阳性，Cardinium阳性）。然后，我们分别测定了W-C+和W+C+蜘蛛的脂肪含量和游离氨基酸含量。我们还检测了不同寄主年龄禾稻瘟病菌的转录组以及脂肪和氨基酸代谢相关基因的表达。结果表明，W+C+雄蛛脂肪含量高于W-C+雄蛛，游离氨基酸含量高于W-C+雄蛛，雌蛛无差异。转录组分析鉴定出144个（W-C+ vs W+C+）差异表达基因（deg）。此外，W+C+蜘蛛的脂肪和氨基酸代谢相关的5个基因的表达在3、4、5龄阶段显著上调。本研究表明沃尔巴克氏体与红柱头菌共同感染对宿主体内脂肪和氨基酸合成有关键影响。此外，我们的研究结果为解释宿主和内共生生物的长期共存提供了强有力的证据。
Associations between endosymbiotic bacteria and their hosts are widespread in nature and have been demonstrated extensively; however, only a few studies have examined how facultative symbionts affect host nutrition and metabolism. To gain insight into the associations between facultative symbionts and host nutrition and metabolic activity, we detected endosymbiotic infection in a small spider species, Hylyphantes graminicola, and established two infectious strains, i.e., W-C+ (Wolbachia negative, Cardinium positive) and W+C+ (Wolbachia positive, Cardinium positive). We then determined the content of fat and free amino acids in W-C+ and W+C+ spiders, respectively. We also detected the transcriptome of H. graminicola and the expression of genes involved in fat and amino acid metabolism at different host ages. Results showed that fat content in W+C+ spiders was higher than that in W-C+ spiders, and free amino acid content was higher in W+C+ males than W-C+ males, with no difference observed in females. Transcriptome analysis identified 144 (W-C+ vs W+C+) differentially expressed genes (DEGs). Moreover, the expression of five genes involved in fat and amino acid metabolism were significantly up-regulated in the third, fourth, and fifth instar stages in W+C+ spiders. This study indicated that Wolbachia and Cardinium co-infection had a pivotal effect on fat and amino acid synthesis in hosts. Moreover, our results provide strong evidence explaining the long-term coexistence of hosts and endosymbionts.