THE ZETA-CHAIN IS ASSOCIATED WITH A TYROSINE KINASE AND UPON T-CELL ANTIGEN RECEPTOR STIMULATION ASSOCIATES WITH ZAP-70, A 70-KDA TYROSINE PHOSPHOPROTEIN
THE ZETA-CHAIN IS ASSOCIATED WITH A TYROSINE KINASE AND UPON T-CELL ANTIGEN RECEPTOR STIMULATION ASSOCIATES WITH ZAP-70, A 70-KDA TYROSINE PHOSPHOPROTEIN
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DOI：
10.1073/pnas.88.20.9166
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
WEISS, A
中科院分区：
文献类型：
--
作者：
CHAN, AC;IRVING, BA;WEISS, A
T细胞抗原受体（TCR）的刺激导致许多细胞蛋白的酪氨酸磷酸化，包括磷脂酶C（PLC）γ-1和TCR ζ链。我们在这里描述了一个70 kDa的酪氨酸磷蛋白（ZAP-70），与ζ在TCR刺激后15秒内。ZAP-70的磷酸化及其与zeta的结合不依赖于其它TCR链，因为TCR阴性T细胞中功能性CD 8/zeta嵌合受体的刺激导致ZAP-70与嵌合蛋白的共沉淀。在表达TCR和CD 8/zeta嵌合蛋白的Jurkat细胞中，ZAP-70的酪氨酸磷酸化和缔合仅与受刺激的受体复合物一起发生。此外，酪氨酸激酶，似乎不fyn协会与zeta和磷酸化zeta和ZAP-70在体外的胞质结构域。
Stimulation of the T-cell antigen receptor (TCR) leads to tyrosine phosphorylation of a number of cellular proteins, including phospholipase C (PLC) gamma-1 and the TCR zeta-chain. We describe here a 70-kDa tyrosine phosphoprotein (ZAP-70) that associates with zeta within 15 sec following TCR stimulation. The phosphorylation of ZAP-70 and its association with zeta is independent of the other TCR chains since stimulation of a functional CD8/zeta chimeric receptor in a TCR-negative T cell leads to coprecipitation of ZAP-70 with the chimeric protein. In a Jurkat cell expressing the TCR and the CD8/zeta chimeric protein, tyrosine phosphorylation and association of ZAP-70 occurs exclusively with the stimulated receptor complex. In addition, a tyrosine kinase that does not appear to be fyn associates with the cytoplasmic domain of zeta and phosphorylates-zeta and ZAP-70 in vitro.