Brucella melitensis and B-abortus in eggs, larvae and engorged females of Dermacentor marginatus
Brucella melitensis and B-abortus in eggs, larvae and engorged females of Dermacentor marginatus
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DOI：
10.1016/j.ttbdis.2018.03.021
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发表时间：
2018-05-01
影响因子：
3.2
通讯作者：
Wang, Yuanzhi
中科院分区：
文献类型：
--
作者：
Wang, Qian;Zhao, Shanshan;Wang, Yuanzhi
布鲁氏菌病是一种比较常见的人畜共患病，特别是在发展中国家。我们的目的是培养布鲁氏菌野生型菌株从鸡蛋的边缘革蜱，并在分子上研究布鲁氏菌DNA的存在下，在不同的发展阶段。从绵羊和牛中收集了总共350只饱食的成年雌性蜱，并将其单独置于适合产卵和幼虫发育的单个清洁透气管中。根据布鲁氏菌外膜蛋白基因22（omp 22）和IS 711基因，尝试在饱食的雌性蜱及其后代中检测布鲁氏菌DNA。此外，对布鲁氏菌培养基进行细菌分离。布鲁氏菌DNA阳性的饱食雌蜱的卵为布鲁氏菌omp 22阳性（4.6%，16/350）。从布鲁氏菌DNA阳性卵群发育而来的幼虫感染率为40.9%（90/220）。对IS 711基因进行BLASTn分析，结果表明，10个（4.1%，10/242）PCR产物为羊种布鲁氏菌。108株中有6株（5.6%，6/108）为B型，其中6株为B型。流产D.感染牛的边缘虫。B。melitensis生物型3是从杜氏锥虫卵中分离到的。边缘。这是首次证实布鲁氏菌在D.边缘。布氏杆菌可能部分由D.缘毛杜父鱼卵对幼虫的转化率为40.9%;边缘。这些发现扩展了我们对布鲁氏菌在D. marginatus后代。
Brucellosis is a relatively common zoonosis, particularly in the developing countries. Our aim was to cultivate the Brucella wild-type strain from eggs of Dermacentor marginatus, and to molecularly investigate the presence of Brucella DNA in different developmental stages. A total of 350 engorged adult female ticks were collected from sheep and cattle, and individually placed into a single clean breathable tube suitable for laying eggs and for the development of larvae. Detection of Brucella DNA was attempted in engorged female ticks and their offsprings based on the Brucella outer membrane protein gene 22 (omp22) and IS711 gene. Additionally, bacterial isolation in the culture medium of Brucella was performed. The eggs from the Brucella DNA-positive engorged female ticks were positive for Brucella omp22 (4.6%, 16/350). The prevalence was 40.9% (90/220) in larvae, which developed from the Brucella DNA-positive egg batch. Based on BLASTn analysis of IS711 gene, ten (4.1%, 10/242) PCR products were identified as Brucella melitensis in D. marginatus from sheep, while six (5.6%, 6/108) were identified as B. abortus in D. marginatus infesting cattle. B. melitensis biotype 3 was isolated from eggs of D. marginatus. This is the first study which demonstrates the transovarial transmission of Brucella in the D. marginatus. Furthermore, Brucella may be partially transmitted from D. marginatus eggs to larvae (40.9%) in D. marginatus. These findings extend our knowledge on the transmission of Brucella in D. marginatus offsprings.