ReMOT Control Delivery of CRISPR-Cas9 Ribonucleoprotein Complex to Induce Germline Mutagenesis in the Disease Vector Mosquitoes Culex pipiens pallens (Diptera: Culicidae)
ReMOT Control Delivery of CRISPR-Cas9 Ribonucleoprotein Complex to Induce Germline Mutagenesis in the Disease Vector Mosquitoes Culex pipiens pallens (Diptera: Culicidae)
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DOI：
10.1093/jme/tjab016
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发表时间：
2021-02-16
影响因子：
2.1
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
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作者：
Li, Xixi;Xu, Yang;Zhu, Changliang
淡色库蚊复合体蚊子的广泛分布使得很难阻止蚊媒疾病在人类中的传播。使用 CRISPR/Cas9 进行基因编辑是一种有效的技术，有可能解决日益严重的蚊媒疾病问题。这项研究使用淡色库蚊 (L.) 的 ReMOT 控制技术来生产转基因蚊子。建立显微注射系统，注射60只成年雌蚊，注射液14μl，无沉淀发生。
The wide distribution of Culex (Cx.) pipiens complex mosquitoes makes it difficult to prevent the transmission of mosquito-borne diseases in humans. Gene editing using CRISPR/Cas9 is an effective technique with the potential to solve the growing problem of mosquito-borne diseases. This study uses the ReMOT Control technique in Culex pipiens pallens (L.) to produce genetically modified mosquitoes. A microinjection system was established by injecting 60 adult female mosquitoes-14 mu l injection mixture was required, and no precipitation occurred with