Antibacterial activation of hydroxyapatite (HA) with controlled porosity by different antibiotics
Antibacterial activation of hydroxyapatite (HA) with controlled porosity by different antibiotics
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DOI：
10.1016/j.bioeng.2007.08.001
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发表时间：
2007-11-01
期刊：
影响因子：
--
通讯作者：
Hildebrand, H. F.
中科院分区：
文献类型：
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作者：
Chai, F.;Hornez, J. -C.;Hildebrand, H. F.
为了防止围术期感染频率增加，生物陶瓷可以装载抗菌剂，抗菌剂会根据其化学特性释放。一种新型羟基磷灰石（HA）具有特定的内部孔隙率，可用作骨生物活性抗生素（ATB）载体材料。通过紫外分光光度法和抑菌试验来测试负载不同抗生素后的ATB吸附和微生物有效性。通过接触不同的释放溶剂和不同的病原菌：金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌，研究了影响ATB负载效果的浸渍时间、ATB浸渍浓度、浸渍条件等因素。它清楚地表明，在简单的非真空浸渍条件下，在不太长的浸渍间隔下，在这种多孔 HA 上负载 ATB 的设施甚至是可能的。结果还表明，对于所有三种类型的 ATB（万古霉素、环丙沙星和庆大霉素），微孔 HA 上的吸附量均远高于致密 HA 上的吸附量。即使在模拟生理条件下，测试HA的微孔性也显着延长了抗生素的释放时间。此外，还通过中试初步证明，通过冷等离子体技术等其他功能化方法可以进一步显着提高粗制微孔HA的抗菌效率。 (C) 2007 Elsevier B.V. 保留所有权利。
In order to prevent the increasing frequency of per-operative infections, bioceramics can be loaded with anti-bacterial agents, which will release with respect to their chemical characteristics. A novel hydroxyapatite (HA) was elaborated with specific internal porosities for using as a bone-bioactive antibiotic (ATB) carrier material. UV spectrophotometry and bacteria inhibition tests were performed for testing the ATB adsorption and the microbiological effectiveness after loading with different antibiotics. The impregnation time, ATB impregnating concentration, impregnation condition and other factors, which might influence the ATB loading effect, were studied by exposure to different releasing solvents and different pathogenic bacteria: Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. It clearly showed that the facility of ATB loading on this porous HA is even possible just under simple non-vacuum impregnation conditions in a not-so-long impregnating interval. The results also showed that, for all three types of ATB (vancomycin, ciprofloxacin and gentamicin), adsorbed amount on the micro-porous HA were hugely higher than that on dense HA. The micro-porosity of test HA had also significantly prolonged the release time of antibiotics even under mimic physiological conditions. Furthermore, it also has primarily proved by a pilot test that the antibacterial efficiency of crude micro-porous HA could be further significantly improved by other methods of functionalization such as cold plasma technique. (C) 2007 Elsevier B.V. All rights reserved.