Zooming in on a quantitative trait for tomato yield using interspecific introgressions
Zooming in on a quantitative trait for tomato yield using interspecific introgressions
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DOI：
10.1126/science.1101666
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发表时间：
2004-09-17
期刊：
影响因子：
56.9
通讯作者：
Zamir, D
中科院分区：
文献类型：
--
作者：
Fridman, E;Carrari, F;Zamir, D
为了探索自然生物多样性，我们在栽培番茄（S. lycopersicum）的背景下开发并检查了含有野生物种（Solanum pennellii）染色体片段的基因渗入系（IL）。我们鉴定了 Brix9-2-5，它是潘氏酵母数量性状基因座 (QTL)，可提高番茄的糖产量，并被定位在花和果实特异性转化酶 (LIN5) 内。代表五个不同番茄品种的 QTL 分析将 Brix-9-2-5 的功能多态性界定为靠近蔗糖酶晶体催化位点的氨基酸，影响酶动力学和果实库强度。这些结果强调了多种 IL 在复杂表型高分辨率视角方面的力量。
To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix-9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.