Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)
Rapid verification of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson)
复制标题
白鲟减数分裂雌核发育和多倍体的快速验证（Acipenser transmontanus Richardson）
DOI：
10.1016/s0044-8486(96)01369-5
复制
发表时间：
1996
期刊：
影响因子：
4.5
通讯作者：
S. Doroshov
中科院分区：
文献类型：
--
作者：
Alison L. Van Eenennaam;J. P. Eenennaam;J. Medrano;S. Doroshov
我们描述了一种新的随机扩增多态性DNA（RAPD）为基础的技术，以快速评估设计诱导雌核发育的治疗的整体成功。为了测试这种技术，我们生产了白色鲟鱼（Acipenser transmontanus）减数分裂雌核细胞。共108个假定的雌激素已知的父母从四个不同的实验处理进行了筛选，使用RAPD引物，这是已知的产生sire-specific标记。只有两个人表现出放大的sire-specific标记，表明他们已经收到了一些父系遗传，而不是真正的雌激素。这种简单的基于RAPD的技术可以普遍适用于其他物种的雌核发育或雄核发育的验证，特别是那些缺乏合适的表型标记来追踪亲本遗传传递的物种。同时，对2469尾二倍体、三倍体、四倍体和花叶鲟的红细胞核大小进行了分析，并用流式细胞仪对分析结果进行了验证。库尔特倍性分析仪是一种快速、准确的鲟鱼倍性分析方法。
We describe a novel random amplified polymorphic DNA (RAPD) based technique to rapidly assess the overall success of treatments designed to induce gynogenesis. To test this technique we produced white sturgeon (Acipenser transmontanus) meiotic gynogens. A total of 108 putative gynogens of known parentage from four different experimental treatments were screened using RAPD primers which were known to generate sire-specific markers. Only two individuals showed amplification of sire-specific markers indicating that they had received some paternal inheritance and were not true gynogens. This simple RAPD-based technique could be generally applicable for the verification of gynogenesis or androgenesis in other species, especially those which lack suitable phenotypic markers to trace the transmission of parental inheritance. We also determined the ploidy of 2469 diploid, triploid, tetraploid and mosaic sturgeon by using a Coulter Counter to analyze erythrocyte nuclei size and verified the results with flow cytometry. The Coulter Counter proved to be a rapid and accurate technique for ploidy analysis in sturgeon.
DOI：
10.1002/cyto.990100606
发表时间：
1989-11-01
期刊：
CYTOMETRY
影响因子：
--
作者：
TIERSCH, TR;CHANDLER, RW;ELIAS, S
通讯作者：
ELIAS, S