THE GENE FAMILY ENCODING EGGSHELL PROTEINS OF SCHISTOSOMA-JAPONICUM
THE GENE FAMILY ENCODING EGGSHELL PROTEINS OF SCHISTOSOMA-JAPONICUM
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DOI：
10.1016/0166-6851(90)90114-2
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发表时间：
1990-08-01
影响因子：
1.5
通讯作者：
DONELSON, JE
中科院分区：
文献类型：
--
作者：
HENKLE, KJ;COOK, GA;DONELSON, JE
描述了人类寄生虫日本血吸虫（Schistosoma japonicum）中编码蛋壳蛋白的四个密切相关的基因。构建了一个cDNA和基因组DNA文库，并分离了蛋壳蛋白基因家族的成员。该家族中的四个基因不含内含子，并且在组织和核苷酸序列上与曼氏血吸虫和埃及血吸虫中的相关基因组不同。对其中两个S.日本血吸虫基因及其侧翼区。引物延伸分析显示，这些基因的转录起始位点出现在起始密码子前面的47和50个核苷酸处。核提取物中的雌性特异性组分与转录起始位点上游含有保守序列的DNA片段结合。推导出的蛋白质序列由207个和212个氨基酸组成，其中50%是甘氨酸，连续的甘氨酸区域长达11个残基。雄性和雌性RNA的体外翻译揭示了雌性特异性翻译产物，其大小与蛋壳蛋白一致。
The four closely related genes encoding eggshell proteins in the human parasite Schistosoma japonicum are described. A cDNA and a genomic DNA library were constructed and members of the eggshell protein gene family isolated. The four genes in this family do not contain introns, and differ in organization and nucleotide sequence from the related set of genes in Schistosoma mansoni and Schistosoma haematobium. The coding sequences of two of the S. japonicum genes and their flanking regions were determined. Transcription start sites for these genes were shown by primer extension analysis to occur 47 and 50 nucleotides in front of the start codon. A female-specific component in nuclear extracts binds to a DNA fragment containing conserved sequences upstream of the transcription start sites. The deduced protein sequences of 207 and 212 amino acids are composed of 50% glycine with continuous glycine regions as long as 11 residues. In vitro translations of male and female RNAs revealed female-specific translation products, the sizes of which were consistent with the eggshell proteins.