Identification of Legionella Species by Photogate-Type Optical Sensor
Identification of Legionella Species by Photogate-Type Optical Sensor
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DOI：
10.1109/sensors43011.2019.8956717
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发表时间：
2019-10
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影响因子：
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通讯作者：
S. Tanaka;S. Yoshida;Y. Choi;T. Noda;M. Ishida;K. Sawada;H. Ishii;K. Machida;Yasuhiko Nikaido;M. Saito
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文献类型：
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作者：
S. Tanaka;S. Yoshida;Y. Choi;T. Noda;M. Ishida;K. Sawada;H. Ishii;K. Machida;Yasuhiko Nikaido;M. Saito
这项工作描述了使用光栅型光学传感器（PG 传感器）和 MEMS 芯片来检测军团菌细菌的简单方法。我们观察了杜莫菲军团菌 (L. dumoffii) 和红斑军团菌 (L. erythra) 在暴露于紫外线时的荧光行为。用PG传感器获得的杜莫菲军团菌和红斑军团菌的荧光强度的时间依赖性与用光谱仪获得的荧光强度的时间依赖性非常吻合，表明每种军团菌都能够被PG传感器检测到。利用PG传感器和光学滤光片的组合，利用它们行为的差异，成功地对杜莫氏乳杆菌和红斑乳杆菌的混合细菌系统中的每种细菌进行了鉴定。
This work describes simple method for sensing bacteria, Legionella by using a photogate-type optical sensor (PG-sensor) and MEMS chip. We observed the behavior of fluorescence from Legionella dumoffii (L. dumoffii) and Legionella erythra (L. erythra) when they are exposed to ultraviolet light. The time dependence of the fluorescence intensity of L. dumoffii and L. erythra obtained with PG-sensor agreed well with that of fluorescence intensity obtained with spectrometer, showing that each Legionella is able to be detected by PG-sensor. Using the difference of their behavior, the identification of each bacterium was successfully performed for a mixed bacterial system of L. dumoffii and L. erythra by using the combination of PG-sensor and optical filters.