A joint transcriptomic, proteomic and metabolic analysis of maize endosperm development and starch filling.
A joint transcriptomic, proteomic and metabolic analysis of maize endosperm development and starch filling.
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DOI：
10.1111/j.1467-7652.2008.00368.x
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发表时间：
2008-12
影响因子：
13.8
通讯作者：
J. Prioul;V. Méchin;P. Lessard;C. Thévenot;M. Grimmer;S. Chateau-Joubert;S. Coates;H. Hartings
中科院分区：
文献类型：
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作者：
J. Prioul;V. Méchin;P. Lessard;C. Thévenot;M. Grimmer;S. Chateau-Joubert;S. Coates;H. Hartings
研究了玉米胚乳转录体的三个特征时期：(1)滞后期[授粉后10天(DAP)]；(2)开始贮藏(14天)；(3)最大淀粉积累速率(21天)。分别获得711、757和384个相关克隆的表达序列标签，并手动检查。没有明确功能的序列比例从35%下降到20%，贮藏蛋白序列从阶段(I)到阶段(III)大幅增加(即5%到38%)。其余主要类别包括代谢(11%-13%)、转录-RNA加工-蛋白质合成(13%-20%)、蛋白质目的地(5%-9%)、细胞通讯(3%-9%)和细胞拯救-防御(4%)。10-DAP转录组中的类别等级与最近报道的14-DAP蛋白质组之间的一致性很好，但在后者中没有检测到用于RNA加工的激酶和蛋白质。在代谢类中，呼吸途径的转录物所占比例最大(25%-37%)，并在21DAP时显示出有利于糖酵解的转变。在这一阶段，氨基酸代谢增加到17%，而淀粉代谢出人意料地下降到7%。第二个实验侧重于碳水化合物代谢，通过比较三个水平(转录本、蛋白质和酶活性)的基因表达与底物或产物之间的关系，从10DAP到40DAP。这里观察到两种不同的模式：转化酶和己糖在开始时占主导地位，而淀粉途径中的酶模式在三个水平上预期并平行于淀粉积累，这表明在大多数情况下，转录调控负责调节淀粉的生物合成。
The maize endosperm transcriptome was investigated through cDNA libraries developed at three characteristic stages: (i) lag phase [10 days after pollination (DAP)]; (ii) beginning of storage (14 DAP); and (iii) maximum starch accumulation rate (21 DAP). Expressed sequence tags for 711, 757 and 384 relevant clones, respectively, were obtained and checked manually. The proportion of sequences with no clear function decreased from 35% to 20%, and a large increase in storage protein sequences (i.e. 5% to 38%) was observed from stages (i) to (iii). The remaining major categories included metabolism (11%-13%), transcription-RNA processing-protein synthesis (13%-20%), protein destination (5%-9%), cellular communication (3%-9%) and cell rescue-defence (4%). Good agreement was generally found between category rank in the 10-DAP transcriptome and the recently reported 14-DAP proteome, except that kinases and proteins for RNA processing were not detected in the latter. In the metabolism category, the respiratory pathway transcripts represented the largest proportion (25%-37%), and showed a shift in favour of glycolysis at 21 DAP. At this stage, amino acid metabolism increased to 17%, whereas starch metabolism surprisingly decreased to 7%. A second experiment focused on carbohydrate metabolism by comparing gene expression at three levels (transcripts, proteins and enzyme activities) in relation to substrate or product from 10 to 40 DAP. Here, two distinct patterns were observed: invertases and hexoses were predominant at the beginning, whereas enzyme patterns in the starch pathway, at the three levels, anticipated and paralleled starch accumulation, suggesting that, in most cases, transcriptional control is responsible for the regulation of starch biosynthesis.