TRANSFER OF MYELOMA IDIOTYPE-SPECIFIC IMMUNITY FROM AN ACTIVELY IMMUNIZED MARROW DONOR
TRANSFER OF MYELOMA IDIOTYPE-SPECIFIC IMMUNITY FROM AN ACTIVELY IMMUNIZED MARROW DONOR
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DOI：
10.1016/s0140-6736(95)90757-2
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发表时间：
1995-04-22
期刊：
影响因子：
168.9
通讯作者：
LONGO, DL
中科院分区：
文献类型：
--
作者：
KWAK, LW;TAUB, DD;LONGO, DL
骨髓瘤免疫球蛋白的独特型可用作独特的肿瘤特异性抗原。我们检验了肿瘤抗原特异性免疫可以从骨髓移植供体转移到受体的假设，我们在骨髓移植前用来自受体血浆的骨髓瘤免疫球蛋白免疫健康的同胞供体，骨髓瘤免疫球蛋白与免疫原性载体蛋白结合并在佐剂中乳化。检测到淋巴增殖反应、载体蛋白中的平行反应、对骨髓瘤独特型具有独特特异性的受体CD 4 + T细胞系的回收，并通过原位杂交证明细胞系为供体来源，证明了骨髓瘤独特型特异性T-骨髓瘤独特型供体免疫可能代表了一种新的策略，异基因骨髓移植物的特异性抗肿瘤作用
The idiotype of myeloma immunoglobulin can be used as a unique tumour-specific antigen. We tested the hypothesis that tumour antigen-specific immunity can be transferred from bone-marrow-transplant donor to recipient.We immunised a healthy sibling donor with myeloma immunoglobulin from the plasma of the recipient, conjugated to an immunogenic carrier protein and emulsified in an adjuvant, before marrow transplantation. Detection of a lymphoproliferative response, a parallel response in the carrier protein, recovery of a recipient CD4+ T-cell line with unique specificity for myeloma idiotype, and demonstration by in-situ hybridisation that the cell line was of donor origin, proved that a myeloma idiotype-specific T-cell response was successfully transferred to the recipient.Donor immunisation with myeloma idiotype may represent a new strategy for enhancing the specific antitumour effect of allogeneic marrow grafts.