Glucocorticoid Receptors of Normal Human Epidermis
Glucocorticoid Receptors of Normal Human Epidermis
复制标题
正常人表皮的糖皮质激素受体
DOI：
10.1111/1523-1747.ep12506283
复制
发表时间：
1982
影响因子：
6.5
通讯作者：
J. Bonifas
中科院分区：
文献类型：
--
作者：
E. Epstein;J. Bonifas
成人表皮细胞质可与地塞米松结合，且与其他组织糖皮质激素受体的结合相似，可从结合的亲和力、结合的特异性、受体-类固醇复合物的分子大小、复合物与细胞核的结合能力4个标准来判断。为了证明这些受体，表皮可以通过胰蛋白酶化分离，但只有在皮肤与钼酸盐初步孵育后才能分离，这可以防止受体在加热到22°C时失活。这些受体的存在表明糖皮质激素可能直接作用于表皮。五种局部使用的糖皮质激素在体外均使3h -地塞米松脱离受体，但这种结合的亲和力与体内使用时类固醇的效力不完全相关。
Epidermal cytosol of adult humans can bind dexamethasone, and this binding resembles that by glucocorticoid receptors of other tissues as judged by 4 criteria: affinity of binding, specificity of binding, molecular size of receptor-steroid complexes, and ability of these complexes to bind to nuclei. For demonstration of these receptors, epidermis can be separated by trypsinization but only after preliminary incubation of the skin with molybdate ion, which prevents inactivation of receptors when heated to 22°C. The presence of these receptors suggest that glucocorticoids may act directly on the epidermis. Five glucocorticoids that are used topically all displaced 3 H-dexamethasone from the receptor in vitro but the affinity of this binding correlates imperfectly with the potency of the steroids when used in vivo .