Genetic Access to Gustatory Disgust-Associated Neurons in the Interstitial Nucleus of the Posterior Limb of the Anterior Commissure in Male Mice
Genetic Access to Gustatory Disgust-Associated Neurons in the Interstitial Nucleus of the Posterior Limb of the Anterior Commissure in Male Mice
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DOI：
10.1016/j.neuroscience.2019.06.021
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发表时间：
2019-08-10
期刊：
影响因子：
3.3
通讯作者：
Tanabe, Tsutomu
中科院分区：
文献类型：
--
作者：
Tanaka, Daisuke H.;Li, Shusheng;Tanabe, Tsutomu
口腔内注射苦奎宁(天生的厌恶)和先前与疾病(习得性厌恶)配对的甜糖精后，大鼠口腔面部和躯体的厌恶反应被观察到。然而，目前尚不清楚这些先天和后天的厌恶反应是否有共同的神经基础，以及大脑的哪些区域(如果有的话)拥有这种反应。此外，还没有既定的方法来从基因上获得其放电与厌恶相关的神经元(厌恶相关神经元)。在这里，我们检测了CFos和Arc的表达，这两个神经元活动的标志物在表现出先天厌恶的雄性小鼠和表现出后天厌恶的小鼠的前连合后肢(IPAC)的间质核中。此外，我们使用了一种活性群体中的定向重组(TRAP)方法来用YFP对IPAC中与厌恶相关的神经元进行遗传标记。我们发现，在表现出先天厌恶和习得性厌恶的小鼠的IPAC中，CFos阳性神经元和Arc阳性神经元都显着增加。此外，奎宁注射后的TRAP(奎宁-TRAP)与注水后的TRAP相比，在IPAC中导致更多的YFP阳性神经元。奎宁-TRAP后大量的YFP阳性神经元在第二次奎宁注射后与Arc共标记，证实了奎宁-TRAP在IPAC中优先标记奎宁激活的神经元。我们的结果表明，IPAC的活动既与先天的厌恶有关，也与后天的厌恶有关，而且IPAC中与厌恶相关的神经元是通过TRAP从基因上获得的。(C)2019 IBRO。爱思唯尔有限公司出版。保留所有权利。
Orofacial and somatic disgust reactions are observed in rats following intraoral infusion of not only bitter quinine (innate disgust) but also sweet saccharin previously paired with illness (learned disgust). It remains unclear, however, whether these innate and learned disgust reactions share a common neural basis and which brain regions, if any, host it. In addition, there is no established method to genetically access neurons whose firing is associated with disgust (disgust-associated neurons). Here, we examined the expression of cFos and Arc, two markers of neuronal activity, in the interstitial nucleus of the posterior limb of the anterior commissure (IPAC) of male mice that showed innate disgust and mice that showed learned disgust. Furthermore, we used a targeted recombination in active populations (TRAP) method to genetically label the disgust-associated neurons in the IPAC with YFP. We found a significant increase of both cFos-positive neurons and Arc-positive neurons in the IPAC of mice that showed innate disgust and mice that showed learned disgust. In addition, TRAP following quinine infusion (Quinine-TRAP) resulted in significantly more YFP-positive neurons in the IPAC, compared to TRAP following water infusion. A significant number of the YFP-positive neurons following Quinine-TRAP were co-labeled with Arc following the second quinine infusion, confirming that Quinine-TRAP preferentially labeled quinine-activated neurons in the IPAC. Our results suggest that the IPAC activity is associated with both innate and learned disgust and that disgust-associated neurons in the IPAC are genetically accessible by TRAP. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.