Molecular NMR T2 values can predict cartilage stress-relaxation parameters.
Molecular NMR T2 values can predict cartilage stress-relaxation parameters.
复制标题
分子 NMR T2 值可以预测软骨应力松弛参数。
DOI：
10.1016/j.bbrc.2008.09.067
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发表时间：
2008
影响因子：
3.1
通讯作者：
Fyhrie,DavidP
中科院分区：
文献类型：
--
作者：
June,RonaldK;Fyhrie,DavidP
关节软骨连接滑膜关节，作为一种低摩擦的可变形组织，使关节关节平滑稳定。本研究的目的是确定软骨应力松弛特性与胶原蛋白和GAG NMR横向松弛时间（T2）之间的关系，以了解软骨粘弹性的机制。对培养的和酶消化的牛软骨进行应力松弛测试，然后使用Call-Purcell-Meiboom-Gill脉冲序列测量胶原蛋白和GAG 2。峰值应力和平衡应力与GAG T2相关，应力松弛时间常数与胶原T2相关。多元线性回归模型成功地利用特定的2值来预测应力松弛特性。作为骨关节炎模型，胶原酶和睾丸透明质酸酶酶消化对2值的影响较弱。这些数据呈现了软骨力学行为的复杂图像，软骨刚度与GAG T2值相关，应力松弛时间常数与胶原T2相关。
Articular cartilage lines synovial joints and functions as a low-friction deformable tissue to enable smooth and stable joint articulation. The objective of this study was to determine the relationships between cartilage stress-relaxation properties and the collagen and GAG NMR transverse relaxation times (T2) toward understanding mechanisms of cartilage viscoelasticity. Stress-relaxation tests were performed on both cultured and enzymatically digested bovine cartilage, followed by measurements of both the collagen and GAG T2using the Call–Purcell–Meiboom–Gill pulse sequence. The peak and equilibrium stresses were correlated with the GAG T2, and the stress-relaxation time constant was correlated with the collagen T2. Multiple linear regression models were successful in using the specific T2values to predict the stress-relaxation properties. As a model of osteoarthritis, enzymatic digestion with collagenase and testicular hyaluronidase had weak effects on T2values. These data present a complex picture of cartilage mechanical behavior, with cartilage stiffness associated with the GAG T2values and the stress-relaxation time constant associated with the collagen T2.