Stimulation of differentiation and proliferation of haemopoietic cells in vitro
Stimulation of differentiation and proliferation of haemopoietic cells in vitro
复制标题
体外刺激造血细胞的分化和增殖
DOI：
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发表时间：
1973
影响因子：
5.6
通讯作者：
B. Lord
中科院分区：
文献类型：
--
作者：
T. Dexter;T. Allen;L. Lajtha;R. Schofield;B. Lord
A liquid culture system, for haemopoietic cells, has been developed using bone marrow cells alone, or co‐cultures of thymus and bone marrow cells, inoculated into four ounce medical bottles. After several days growth, such cultures consisted of an attaching population of cells, forming discrete colonies, and a non‐attaching population. In the (co‐cultures) there was a 2 X enhancement of monolayer colony development compared with the combined total present in the (marrow alone) plus (thymus alone) cultures. Also, better maintenance of non‐attaching cells was seen in the (co‐cultures). Normal CFUS and CFUC were present in both the (marrow alone) and the (co‐cultures) for at least 14 days.
