GAC1 MAY ENCODE A REGULATORY SUBUNIT FOR PROTEIN PHOSPHATASE TYPE-1 IN SACCHAROMYCES-CEREVISIAE
GAC1 MAY ENCODE A REGULATORY SUBUNIT FOR PROTEIN PHOSPHATASE TYPE-1 IN SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1002/j.1460-2075.1992.tb05031.x
复制
发表时间：
1992-01-01
期刊：
影响因子：
11.4
通讯作者：
TATCHELL, K
中科院分区：
文献类型：
--
作者：
FRANCOIS, JM;THOMPSONJAEGER, S;TATCHELL, K
来自酿酒酵母的GAC 1基因的高剂量引起糖原的过度积累，而GAC 1的基因破坏导致糖原水平降低。在具有增加的GAC 1基因剂量的细胞中，糖原合酶几乎完全处于活性、不依赖于葡萄糖6-磷酸的形式，而在缺乏GAC 1的菌株中，该酶大部分处于失活形式。GAC 1编码一种88 kDa的蛋白质，该蛋白质类似于来自骨骼肌的磷蛋白磷酸酶1型（PP-1）的调节亚基（R（G1）），其将PP-1靶向糖原颗粒。综上所述，这些结果表明GAC 1编码PP-1的调节亚基。如先前对于糖原磷酸化酶（GPH 1）所示，GAC 1 RNA随着糖原的出现而累积。在cAMP依赖性蛋白激酶（bcy 1）的调节亚基中具有突变的菌株不能积累GPH 1和GAC 1 RNA。这些结果表明，协调调节参与糖原代谢的酶在RNA积累的水平，并表明，至少有一部分的控制是由RAS-cAMP途径。
Elevated dosage of the GAC1 gene from the yeast Saccharomyces cerevisiae causes hyperaccumulation of glycogen whereas a gene disruption of GAC1 results in reduced glycogen levels. Glycogen synthase is almost entirely in the active, glucose 6-phosphate-independent, form in cells with increased gene dosage of GAC1 whereas the enzyme is mostly in the inactive form in strains lacking GAC1. GAC1 encodes an 88 kDa protein that is similar to the regulatory subunit (R(G1)) of phosphoprotein phosphatase type 1 (PP-1) from skeletal muscle that targets PP-1 to glycogen particles. Taken together, these results suggest that GAC1 encodes a regulatory subunit of PP-1. As previously shown for glycogen phosphorylase (GPH1), GAC1 RNA accumulates concomitantly with the appearance of glycogen. A strain with a mutation in the regulatory subunit of the cAMP-dependent protein kinase (bcy1) fails to accumulate GPH1 and GAC1 RNA. These results point to coordinate regulation of enzymes involved in glycogen metabolism at the level of RNA accumulation and indicate that at least part of this control is exerted by the RAS-cAMP pathway.