Retinal ganglion cells with distinct directional preferences differ in molecular identity, structure, and central projections.
Retinal ganglion cells with distinct directional preferences differ in molecular identity, structure, and central projections.
复制标题
DOI：
10.1523/jneurosci.0907-11.2011
复制
发表时间：
2011-05-25
期刊：
影响因子：
--
通讯作者：
Sanes JR
中科院分区：
文献类型：
--
作者：
Kay JN;De la Huerta I;Kim IJ;Zhang Y;Yamagata M;Chu MW;Meister M;Sanes JR
视网膜含有神经节细胞（RGC），其选择性地对在特定方向上移动的物体作出反应。一组ON-OFF方向选择性RGCs（ooDSGCs）的个体成员检测四个方向之一的刺激：腹侧，背侧，鼻侧或颞侧。尽管有这种生理多样性，但对亚型特异性的结构、分子身份和预测差异知之甚少。为了寻求这样的差异，我们的特点是小鼠转基因品系，选择性地标记ooDSGCs喜欢腹侧或鼻运动，以及一条线，标志着腹侧和背侧偏好的子集。然后，我们使用这些细胞系来鉴定细胞表面分子，包括钙粘蛋白6、胶原蛋白25 a1和基质金属蛋白酶17，这些分子由ooDSGCs的不同亚群选择性表达。我们还确定了一种神经肽，CART，区分所有ooDSGCs从其他RGC。总之，这组内源性和转基因标志物区分了四个ooDSGC亚组。分子多样化的模式发生在睁开眼睛之前，因此与经验无关。它们可能有助于解释这四个子集如何获得不同的输入。我们还展示了视网膜内的树突模式和它们向大脑的轴突投射的子集之间的差异。投影的差异表明，关于不同方向上的运动的信息被发送到不同的目的地。
The retina contains ganglion cells (RGCs) that respond selectively to objects moving in particular directions. Individual members of a group of ON-OFF direction-selective RGCs (ooDSGCs) detect stimuli moving in one of four directions: ventral, dorsal, nasal or temporal. Despite this physiological diversity, little is known about subtype-specific differences in structure, molecular identity and projections. To seek such differences, we characterized mouse transgenic lines that selectively mark ooDSGCs preferring ventral or nasal motion as well as a line that marks both ventral- and dorsal-preferring subsets. We then used the lines to identify cell surface molecules, including Cadherin 6, Collagen25a1, and Matrix metalloprotease 17, that are selectively expressed by distinct subsets of ooDSGCs. We also identify a neuropeptide, CART, that distinguishes all ooDSGCs from other RGCs. Together, this panel of endogenous and transgenic markers distinguishes the four ooDSGC subsets. Patterns of molecular diversification occur before eye-opening and are therefore experience-independent. They may help explain how the four subsets obtain distinct inputs. We also demonstrate differences among subsets in their dendritic patterns within the retina and their axonal projections to the brain. Differences in projections indicate that information about motion in different directions is sent to different destinations.