Effect of silodosin, a selective α(1A)-adrenoceptor antagonist, on voiding behavior and bladder blood flow in a rat model of bladder outlet obstruction.
Effect of silodosin, a selective α(1A)-adrenoceptor antagonist, on voiding behavior and bladder blood flow in a rat model of bladder outlet obstruction.
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西洛多辛（一种选择性 α(1A)-肾上腺素受体拮抗剂）对膀胱出口梗阻大鼠模型的排尿行为和膀胱血流量的影响。
DOI：
10.1016/j.ejphar.2015.07.037
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发表时间：
2015
影响因子：
5
通讯作者：
O. Yamaguchi
中科院分区：
文献类型：
--
作者：
Yoshiaki Goi;Y. Tomiyama;Ayaka Yokoyama;S. Tatemichi;K. Maruyama;Mamoru Kobayashi;O. Yamaguchi
本实验旨在研究选择性α 1a -肾上腺素受体拮抗剂西洛多辛对膀胱出口梗阻（BOO）大鼠模型膀胱血流量（BBF）和膀胱功能的影响，并测定α1-肾上腺素受体亚型mRNA在人和大鼠膀胱微血管中的表达。尿道近端部分结扎产生BOO，维持2周。BOO手术后2周，BOO大鼠分别以0.3 mg/kg/天的剂量或通过渗透泵皮下注射西洛多辛。在有意识的动物中进行了代谢笼研究。使用激光散斑血流成像仪测量BBF。测定尿中8-羟基-2′-脱氧鸟苷（8-OHdG）和神经生长因子（NGF）水平。免疫组织学检查大鼠膀胱神经分布及NGF表达。采用原位杂交法检测人和大鼠膀胱微血管中α1-肾上腺素能受体各亚型mRNA的表达。西洛多辛在代谢笼研究中改善了BOO大鼠排尿频率的增加和平均排尿量的减少。西洛多辛也消除了BOO大鼠BBF的下降。西洛多辛可显著降低BOO大鼠8-OHdG和NGF水平。西洛多辛可抑制神经分布的减少和NGF表达的增加。人和大鼠膀胱微血管均表达α1-肾上腺素受体亚型mrna。本研究结果表明西洛多辛通过诱导血脑屏障恢复来改善BOO大鼠模型的排尿行为。
This study was performed to investigate the effects of silodosin (selective α1A-adrenoceptor antagonist) on bladder blood flow (BBF) and bladder function in a rat model of bladder outlet obstruction (BOO) and to determine the expression of α1-adrenoceptor subtype mRNA in human and rat bladder microvessels.BOO was produced by partial ligature of the proximal urethra, which was maintained for 2 weeks. The BOO rats received either silodosin at a rate of 0.3 mg/kg/day or vehicle subcutaneously via an osmotic pump for 2 weeks after BOO surgery. A metabolic cage study was performed in conscious animals. BBF was measured using a Laser Speckle Blood Flow Imager. Urinary levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and nerve growth factor (NGF) were measured. Immunohistological examinations of nerve distribution and NGF expression in the rat bladder were conducted. The expression of each α1-adrenoceptor subtype mRNA in human and rat bladder microvessels was determined by in situ hybridization. Silodosin ameliorated the increase in voiding frequency and decrease in mean voided volume in BOO rats in the metabolic cage study. Silodosin also abrogated the decrease in BBF in BOO rats. The levels of 8-OHdG and NGF in BOO rats were significantly decreased by administration of silodosin. Silodosin prevented the decrease in nerve distribution and increase in NGF expression. Human and rat bladder microvessels showed expression of all α1-adrenoceptor subtype mRNAs. The results presented here suggest that silodosin improves voiding behavior in rat models with BOO by inducing recovery of BBF.
射精障碍与西洛多辛治疗良性前列腺增生的功效增加有关
影响因子：
2.1
作者：
高橋公太、齊藤和英、中川由紀、田崎正之、原昇;他;Isahaya Etsuko;Hara Noboru;Takizawa Itsuhiro;原昇;原昇;原昇;原昇;原昇;原昇;原昇;原昇;原昇;原昇;原昇;原昇;Masayuki Takeda;Yukio Homma
通讯作者：
Yukio Homma