Association of Mre11p with double-strand break sites during yeast meiosis
Association of Mre11p with double-strand break sites during yeast meiosis
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DOI：
10.1016/s1097-2765(04)00034-6
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发表时间：
2004-02-13
期刊：
影响因子：
16
通讯作者：
Nicolas, A
中科院分区：
文献类型：
--
作者：
Borde, V;Lin, W;Nicolas, A
DNA双链断裂（DSB）的修复需要Mre 11/Rad 50/Xrs 2（Nbs 1）复合物的活性。在酿酒酵母中，这种复合物是必需的启动减数分裂重组Spo 11 p催化的程序DSB和断裂端切除，这是必要的同源重组修复。我们报告说，Mre 11 p瞬时协会与染色质的Spo 11依赖DSB地区在整个基因组。突变体分析表明，Mre 11 p结合需要DSB形成所需的所有基因的功能，除了RAD 50。然而，Mre 11 p结合不需要DSB形成本身，因为Mre 11 p瞬时与催化阴性突变体spo 11-Y135 F中的DSB区域相关联。在积累未切除的DSB的突变体中，Mre 11 p从染色质的释放被阻断。我们建议，Mre 11 p是一个组成部分的前DSB复杂的DSB网站上组装，从而确保DSB形成Spo 11 p和处理的休息结束之间的紧密耦合。
The repair of DNA double-strand breaks (DSBs) requires the activity of the Mre11/Rad50/Xrs2(Nbs1) complex. In Saccharomyces cerevisiae, this complex is required for both the initiation of meiotic recombination by Spo11p-catalyzed programmed DSBs and for break end resection, which is necessary for repair by homologous recombination. We report that Mre11p transiently associates with the chromatin of Spo11-dependent DSB regions throughout the genome. Mutant analyses show that Mre11p binding requires the function of all genes required for DSB formation, with the exception of RAD50. However, Mre11p binding does not require DSB formation itself, since Mre11p transiently associates with DSB regions in the catalysis-negative mutant spo11-Y135F. Mre11p release from chromatin is blocked in mutants that accumulate unresected DSBs. We propose that Mre11p is a component of a pre-DSB complex that assembles on the DSB sites, thus ensuring a tight coupling between DSB formation by Spo11p and the processing of break ends.