Measuring the human T cell receptor gamma-chain locus.
Measuring the human T cell receptor gamma-chain locus.
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测量人类 T 细胞受体伽马链基因座。
DOI：
10.1126/science.3498213
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Seidman,JG
中科院分区：
文献类型：
--
作者：
Strauss,WM;Quertermous,T;Seidman,JG
人T细胞受体γ基因座包含11个可变区、5个连接区和2个恒定区片段，共160个酶。在T细胞体细胞发育期间，这些基因通过缺失分隔可变区和连接区的序列而经历重排。通过缺失作图和限制性内切酶图谱分析，确定了该位点的分子图谱。通过标准琼脂糖电泳和场反转电泳解析限制性片段。这些研究表明，在功能性T细胞受体γ链基因形成过程中发生的该位点的缺失长度范围为50至145个酶。这些研究还为理解γ-链肽库的发展提供了结构基础，并扩展了新兴脉冲场电泳技术的潜力。
The human T cell receptor γ locus, including eleven variable-region, five joining-region, and two constant-region segments, is contained in 160 kilobases. During T cell somatic development these genes undergo rearrangement by deletion of the sequences separating the variable and joining regions. The molecular map of this locus was completely defined by deletion mapping and restriction mapping. Restriction fragments were resolved by standard agarose electrophoresis and field inversion electrophoresis. These studies demonstrate that the deletions in this locus, which occur during the formation of a functional T cell receptor γ-chain gene, range from 50 to 145 kilobases in length. These studies also provide a structural basis for understanding the development of the γ-chain peptide repertoire, and extends the potential of the emerging pulsed-field electrophoretic technology.