Alpha-synuclein can function as an antioxidant preventing oxidation of unsaturated lipid in vesicles.
Alpha-synuclein can function as an antioxidant preventing oxidation of unsaturated lipid in vesicles.
复制标题
α-突触核蛋白可以充当抗氧化剂，防止囊泡中不饱和脂质的氧化。
DOI：
10.1021/bi052584t
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Fink,AnthonyL
中科院分区：
文献类型：
--
作者：
Zhu,Min;Qin,Zhi-Jie;Hu,Dongmei;Munishkina,LarissaA;Fink,AnthonyL
α-突触核蛋白是一种与帕金森病相关的突触前蛋白，以胞浆形式和膜结合形式被发现。虽然α-突触核蛋白的功能尚不清楚，但一些观察表明，它与膜的结合是重要的。在本研究中，我们研究了α-突触核蛋白对含有不饱和脂肪酸磷脂的膜中脂质氧化的影响。通过染料C11-BODIPY荧光强度的变化来监测脂质氧化的动力学。我们发现，单体α-突触核蛋白有效地阻止了脂质氧化，而纤维状α-突触核蛋白则没有这种作用。我们的数据表明，α-突触核蛋白防止不饱和脂肪氧化需要它与脂膜结合。质谱学研究表明，α-突触核蛋白的抗氧化作用归因于其通过形成甲硫氨酸亚砜而易于氧化。这些发现提示，α-突触核蛋白抑制脂质氧化可能是该蛋白的一种生理功能。
α-Synuclein, a presynaptic protein associated with Parkinson's disease, is found as both soluble cytosolic and membrane-bound forms. Although the function of α-synuclein is unknown, several observations suggest that its association with membranes is important. In the present study we investigated the effect of α-synuclein on lipid oxidation in membranes containing phospholipids with unsaturated fatty acids. The kinetics of lipid oxidation were monitored by the change in fluorescence intensity of the dye C11-BODIPY. We find that monomeric α-synuclein efficiently prevented lipid oxidation, whereas fibrillar α-synuclein had no such effect. Our data suggest that the prevention of unsaturated lipid oxidation by α-synuclein requires that it bind to the lipid membrane. The antioxidant function of α-synuclein is attributed to its facile oxidation via the formation of methionine sulfoxide, as shown by mass spectrometry. These findings suggest that the inhibition of lipid oxidation by α-synuclein may be a physiological function of the protein.
登录
查看更多内容
影响因子：
2.9
作者：
Volles, MJ;Lee, SJ;Lansbury, PT
通讯作者：
Lansbury, PT
DOI：
10.1016/s0165-3806(96)00210-6
发表时间：
1997-03
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
G. Withers;J. George;G. Banker;D. F. Clayton
通讯作者：
G. Withers;J. George;G. Banker;D. F. Clayton
影响因子：
4.8
作者：
Kubo, S;Nemani, VM;Fortin, DL
通讯作者：
Fortin, DL
影响因子：
2.9
作者：
Narayanan, V;Scarlata, S
通讯作者：
Scarlata, S
影响因子：
2.9
作者：
Michael J. Volles;P. Lansbury
通讯作者：
Michael J. Volles;P. Lansbury