Nanoparticles-based Culture-free Method for Rapid and Sensitive Detection of Staphylococcus aureus in Bloodstream Infections
Nanoparticles-based Culture-free Method for Rapid and Sensitive Detection of Staphylococcus aureus in Bloodstream Infections
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DOI：
10.1016/j.microc.2023.108803
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发表时间：
2023-04
影响因子：
4.8
通讯作者：
Yuting Wang;Jing-ying Xu;Li Cao;Shang Yang;Lin Zhao;Lei He;Zhen Shen;Weiwei Lin;Bishen Shi;Qian Liu;Zhi Huang;Min Li;Hua Wang
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文献类型：
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作者：
Yuting Wang;Jing-ying Xu;Li Cao;Shang Yang;Lin Zhao;Lei He;Zhen Shen;Weiwei Lin;Bishen Shi;Qian Liu;Zhi Huang;Min Li;Hua Wang
金黄色葡萄球菌(S。金黄色葡萄球菌（Aureus）是一种多用途且毒性强的病原体，已成为危及生命的血流感染的主要原因之一，如败血症和心内膜炎。s的发病率和死亡率。近年来，金黄色菌血症仍然很高。s。金黄色葡萄球菌可以更快地启动适当的抗生素治疗，从而降低治疗费用和死亡率。血培养是诊断菌血症的金标准，但耗时且灵敏度低。本文采用万古霉素修饰磁性纳米颗粒（VMPs）和分离胶来富集hs。直接简便地提取金黄色葡萄球菌血中金黄色葡萄球菌，然后用滴数PCR （ddPCR）进行细菌检测。基于纳米颗粒的无培养方法可以浓缩和检测。在3 h内从全血中提取金黄色葡萄球菌，与传统培养方法（3 - 5天）相比，检测时间明显缩短。该方法对金黄色葡萄球菌的检出限低至10 CFU/mL，显著提高了血菌血症的诊断率。开发的vmp - ddpcr策略可能是一种有前途的鉴定工具。在血液感染的早期阶段指导治疗决策，并预测疗效，从而降低死亡率。
Staphylococcus aureus(S. aureus) is a versatile and virulent pathogen and has become one of the leading causes of life-threatening bloodstream infections, such as sepsis and endocarditis. The morbidity and mortality ofS. aureusbacteremia remain high in recent years. Rapid identification ofS. aureuscan lead to faster initiation of appropriate antibiotic treatment, thereby reducing treatment costs and mortality. Blood culture is the gold standard for diagnosing bacteremia, but it is time-consuming and poorly sensitive. Herein, vancomycin modified magnetic nanoparticles (VMPs) and separation glue were introduced to enrichS. aureusin blood in a direct and convenient manner, and then the bacteria were detected by droplet digital PCR (ddPCR). The nanoparticle-based culture-free method can concentrate and detectS. aureusfrom whole blood within 3 h, significantly shortening the detection time compared with the traditional culture method (3–5 days). The limit of detection of this approach for S. aureus was as low as 10 CFU/mL, which significantly improved the diagnostic rate in blood bacteremia. The developed VMPs-ddPCR strategy could be a promising tool for identifyingS. aureus, guiding treatment decisions in the early stages of bloodstream infections, and predicting efficacy and thereby reducing mortality.