Bioinformatics analysis of organizational and expressional characterizations of the IFNs, IRFs and CRFBs in grass carp Ctenopharyngodon idella
Bioinformatics analysis of organizational and expressional characterizations of the IFNs, IRFs and CRFBs in grass carp Ctenopharyngodon idella
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草鱼Ctenopharyngodon idella IFNs、IRFs和CRFBs组织和表达特征的生物信息学分析
DOI：
10.1016/j.dci.2016.03.020
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发表时间：
2016-08-01
影响因子：
2.9
通讯作者：
Su, Jianguo
中科院分区：
文献类型：
--
作者：
Liao, Zhiwei;Wan, Quanyuan;Su, Jianguo
干扰素（IFNs）在抵御病毒感染和细菌入侵的免疫反应中发挥着关键作用。在本研究中，我们系统地鉴定并描述了草鱼（Ctenopharyngodon idella）中的干扰素、其调节因子（干扰素调节因子，IRFs）以及受体（细胞因子受体B家族，CRFBs）。与其他硬骨鱼类一样，草鱼干扰素可分为I型干扰素（IFN - I）和II型干扰素（IFN - II）。IFN - I由两组组成，成熟肽中分别含有两个（I组）或四个（II组）半胱氨酸，并且可进一步分为三个亚组（IFN - a、 - c和 - d），草鱼中包含四个成员：IFN1、IFN2、IFN3、IFN4。IFN - II包含两个成员，即与哺乳动物IFN - γ具有相似性的IFNγ2，以及鲤科鱼类特有的IFNγ1（IFNγ - rel）分子。对干扰素mRNA表达的分析发现，IFN1和IFN - II在许多组织中持续表达，而其他干扰素成员则在特定组织和时间点短暂表达。在免疫反应中，草鱼呼肠孤病毒（GCRV）攻击后，干扰素的转录主要通过多种IRFs进行调控。草鱼中IRF家族拥有13个成员，可进一步分为四个亚家族（IRF - 1、 - 3、 - 4和 - 5亚家族），它们各自通过不同的信号通路在固有免疫和适应性免疫中与干扰素（主要是IFN - I）相互作用，发挥不同的作用。干扰素必须与受体（CRFBs）结合才能发挥其功能。作为干扰素受体的CRFBs在草鱼中有六个成员。我们利用生物信息学工具分析了干扰素、IRFs和CRFBs的结构及表达特征。这些结果可能为干扰素系统的进一步功能研究提供基础数据，并有助于深入理解鱼类抵御病毒感染的免疫机制。（C）2016爱思唯尔有限公司。保留所有权利。
Interferons (IFNs) play crucial roles in the immune response of defense against viral infection and bacteria invasion. In the present study, we systematically identified and characterized the IFNs, their regulatory factors (Interferon Regulatory Factors, IRFs) and receptors (Cytokine Receptor Family B, CRFBs) in grass carp (Ctenopharyngodon idella). Grass carp IFNs can be classified into type I IFN (IFN-I) and type II IFN (IFN-II) like other teleosts. IFN-I consist of two groups with two (group I) or four (group II) cysteines in the mature peptide and can be further divided into three subgroups (IFN-a, -c and -d), containing four members: IFN1, IFN2, IFN3, IFN4 in grass carp. IFN-II contain two members, IFN gamma 2 with the similarity to mammalian IFN gamma and a cyprinid specific IFN gamma 1 (IFN gamma-rel) molecule. mRNA expression analyses of IFNs discovered that IFN1 and IFN-II were sustainably expressed in many tissues, while other IFN members were transiently expressed in specific tissues and time points. In the immune response, IFN transcriptions are primarily regulated through multiple IRFs after grass carp reovirus (GCRV) challenge. IRF family possess thirteen members in grass carp, which can be further divided into four subfamilies (IRF-1, -3, -4 and -5 subfamily), each of them plays different roles in the innate and adaptive immunity via various signaling pathways to interact with IFNs (mainly IFN-I). IFNs have to bind receptors (CRFBs) to perform their functions. CRFBs as IFN receptors contain six members in grass carp. The structure and expression characterizations of IFNs, IRFs and CRFBs were analyzed using bioinformatics tools. These results might provide basic data for the further functional research of IFN system, and deeply understand fish immune mechanisms against virus infection. (C) 2016 Elsevier Ltd. All rights reserved.