Cellular Interactions on Hierarchical Poly(ε-caprolactone) Nanowire Micropatterns
Cellular Interactions on Hierarchical Poly(ε-caprolactone) Nanowire Micropatterns
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DOI：
10.1021/am301013e
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发表时间：
2012-09-01
影响因子：
9.5
通讯作者：
Gan, Zhihua
中科院分区：
文献类型：
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作者：
Du, Ke;Gan, Zhihua
本研究开发了一种双模板法来制备具有高度有序的纳米和微米级形貌的聚（ε - 己内酯）（PCL）分级图案化纳米线。通过改变模板以及PCL薄膜在模板上的熔融时间，可以轻松且良好地控制具有图案化纳米线表面的PCL薄膜的形貌。对具有不同表面结构的PCL薄膜的表面形态、水接触角、蛋白质吸附以及细胞生长行为进行了充分研究。结果表明，PCL纳米线阵列和分级图案化纳米线比表面光滑的PCL薄膜具有更高的蛋白质吸附能力和更好的细胞生长性能。特别是，具有分级纳米线图案的PCL表面最有利于细胞附着和增殖。本研究在制备具有微尺度岛内部纳米线分级结构的聚合物基底方面具有创新性，以深入了解细胞对这种独特形貌的反应，并开发一种构建用于组织工程应用的仿生表面的新方法。
A double template method to fabricate poly(epsilon-caprolactone) (PCL) hierarchical patterned nanowires with highly ordered nano- and microscaled topography was developed in this study. The topography of PCL film with a patterned nanowire surface can be easily and well controlled by changing the template and melting time of PCL film on the templates. The surface morphology, water contact angle, protein adsorption, and cell growth behavior on the PCL films with different surface structures were well studied. The results revealed that the PCL nanowire arrays and the hierarchical patterned nanowires showed higher capability of protein adsorption and better cell growth than the PCL film with smooth surface. Typically, the PCL surface with hierarchical nanowire patterns was most favorable for cell attachment and proliferation. The present study was innovative at fabrication of polymer substrates with hierarchical architecture of nanowires inside microscaled islands to gain insight into the cell response to this unique topography and to develop a new method of constructing the bionic surface for tissue engineering applications.