Solid and liquid obesogenic diets induce obesity and counter-regulatory changes in hypothalamic gene expression in juvenile Sprague-Dawley rats
Solid and liquid obesogenic diets induce obesity and counter-regulatory changes in hypothalamic gene expression in juvenile Sprague-Dawley rats
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DOI：
10.1093/jn/137.6.1483
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发表时间：
2007-06-01
影响因子：
4.2
通讯作者：
Mercer, Julian G.
中科院分区：
文献类型：
--
作者：
Archer, Zoe A.;Corneloup, Jeremie;Mercer, Julian G.
构成成人和儿童饮食的当代食品和饮料几乎肯定会导致肥胖问题。为了建立儿童肥胖模型，我们研究了单独或联合[非纯化对照饮食（C），高能量（HE）或C + HE]与或不与液体补充剂Ensure（EN）一起喂养幼年大鼠2种固体饮食的影响。大鼠喂饲C至4周龄，然后被分配到6个体重匹配的组中的一个，即喂饲C、HE、C + HE、C + EN、HE + EN或C + HE + EN 5周。EN加速体重增加，增加能量摄入和肥胖，无论固体饮食消耗。血清瘦素浓度增加后，消费的所有饮食相比，C组大鼠，但瘦素水平和肥胖之间的解离。固体饮食的类型对下丘脑基因的表达没有影响，除了谷氨酸脱羧酶-67。EN降低弓状核中刺豚鼠相关肽和神经肽Y的mRNA，降低室旁核中DYN的mRNA。强啡肽和CART mRNA在视上视交叉后核减少。下丘脑中食欲信号的减少表明，下丘脑感觉到EN的过度消耗，但任何启动的生理反应不能有效地补偿，并可能被其他系统否定或压倒。提供固体和液体形式的饮食，并有选择，更接近人类环境。了解这些饮食与外周和中枢能量平衡系统之间的相互作用对于揭示人类肥胖及其早期发展的潜在事件至关重要。
Contemporary foods and beverages that constitute the diets of adults and children almost certainly contribute to the obesity problem. To develop a model of childhood obesity, we examined the effects of feeding juvenile rats 2 solid diets, either alone or in combination [nonpurified control diet (C), high-energy (HE), or C+HE] with or without the liquid supplement Ensure (EN). Rats were fed C until 4 wk of age and then were assigned to 1 of 6 weight-matched groups that were fed C, HE, C+HE, C+EN, HE+EN, or C+HE+EN for 5 wk. EN accelerated weight gain and increased energy intake and adiposity irrespective of the solid diet consumed. Serum leptin concentrations were increased after the consumption of all diets when compared with C rats, but there was dissociation between leptin levels and adiposity. The type of solid diet had no effect on the expression of a panel of hypothalamic genes except for glutamate-decarboxylase-67. EN decreased mRNA for agouti-related peptide and neuropeptide Y in the arcuate nucleus and DYN in the paraventricular nucleus. Dynorphin and CART mRNA were decreased in the supraoptic retrochiasmatic nucleus. The reduction in orexigenic signaling in the hypothalamus suggests that overconsumption of EN is sensed by the hypothalamus but that any initiated physiological responses fail to compensate effectively and may be negated or overwhelmed by other systems. Providing diets in solid and liquid form, with choice, mimics more closely the human environment. Understanding the interactions between these diets and peripheral and central energy balance systems could be crucial in unraveling the events underlying human obesity and its early development.