The periodic table of ribonucleotide reductases.
The periodic table of ribonucleotide reductases.
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DOI：
10.1016/j.jbc.2021.101137
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Boal AK
中科院分区：
文献类型：
--
作者：
Ruskoski TB;Boal AK
In most organisms, transition metal ions are necessary cofactors of ribonucleotide reductase (RNR), the enzyme responsible for biosynthesis of the 2′-deoxynucleotide building blocks of DNA. The metal ion generates an oxidant for an active site cysteine (Cys), yielding a thiyl radical that is necessary for initiation of catalysis in all RNRs. Class I enzymes, widespread in eukaryotes and aerobic microbes, share a common requirement for dioxygen in assembly of the active Cys oxidant and a unique quaternary structure, in which the metallo- or radical-cofactor is found in a separate subunit, β, from the catalytic α subunit. The first class I RNRs, the class Ia enzymes, discovered and characterized more than 30 years ago, were found to use a diiron(III)-tyrosyl-radical Cys oxidant. Although class Ia RNRs have historically served as the model for understanding enzyme mechanism and function, more recently, remarkably diverse bioinorganic and radical cofactors have been discovered in class I RNRs from pathogenic microbes. These enzymes use alternative transition metal ions, such as manganese, or posttranslationally installed tyrosyl radicals for initiation of ribonucleotide reduction. Here we summarize the recent progress in discovery and characterization of novel class I RNR radical-initiating cofactors, their mechanisms of assembly, and how they might function in the context of the active class I holoenzyme complex.
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影响因子：
2.9
作者：
Artin, Erin;Wang, Jun;Lohman, Gregory J. S.;Yokoyama, Kenichi;Yu, Guixue;Griffin, Robert G.;Bar, Galit;Stubbe, JoAnne
通讯作者：
Stubbe, JoAnne
DOI：
10.1073/pnas.1811993115
发表时间：
2018-10-02
影响因子：
11.1
作者：
Blaesi EJ;Palowitch GM;Hu K;Kim AJ;Rose HR;Alapati R;Lougee MG;Kim HJ;Taguchi AT;Tan KO;Laremore TN;Griffin RG;Krebs C;Matthews ML;Silakov A;Bollinger JM Jr;Allen BD;Boal AK
通讯作者：
Boal AK
影响因子：
2.9
作者：
Seyedsayamdost, Mohammad R.;Yee, Cyril S.;Stubbe, JoAnne
通讯作者：
Stubbe, JoAnne
影响因子：
2.9
作者：
CLIMENT, I;SJOBERG, BM;HUANG, CY
通讯作者：
HUANG, CY
DOI：
10.1073/pnas.1112715108
发表时间：
2011-12-27
影响因子：
11.1
作者：
Ando, Nozomi;Brignole, Edward J.;Drennan, Catherine L.
通讯作者：
Drennan, Catherine L.