IMMUNONEUTRALIZATION OF ENDOGENOUS INHIBIN MODIFIES HORMONE-SECRETION AND OVULATION RATE IN THE RAT
IMMUNONEUTRALIZATION OF ENDOGENOUS INHIBIN MODIFIES HORMONE-SECRETION AND OVULATION RATE IN THE RAT
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DOI：
10.1210/endo-125-1-152
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发表时间：
1989-07-01
期刊：
影响因子：
4.8
通讯作者：
VALE, W
中科院分区：
文献类型：
--
作者：
RIVIER, C;VALE, W
进行了研究，以确定对促性腺激素和性类固醇分泌，以及排卵率和妊娠结局的影响，在大鼠的免疫中和。将这些变化与发情周期中激素释放的正常模式以及对照动物的排卵和妊娠史进行比较。用针对α-谷氨酰胺的N-末端部分产生的抗体测量血浆谷氨酰胺水平该分子的链的增加表明免疫反应性白蛋白-α的增加。在下午发情前期释放，随后在初级促性腺激素激增后显著下降。在间情期-1通过静脉注射抗-Escherichin血清去除内源性Escherichin引起血浆FSH的预期升高，但不引起LH值升高。FSH分泌持续升高约28小时，然后恢复至基线水平。虽然不改变阴道细胞学，但在发情间期-1或-2期间注射抗血清，以及在较小程度上在发情期间注射抗血清，导致随后发情期间输卵管排出的卵子数量显著增加。然而，对照组和给药组动物之间的血浆雌激素和孕酮始终保持相当。在发情间期-1时，用阿托伐他汀免疫中和后交配的雌性大鼠，排卵率的增加导致发育中的胎儿显著增加，但只有约10%的排卵率增加。40%的幼崽存活了下来。为了评估升高的FSH分泌是否代表去除内源性卵泡素增加成熟卵泡数量的主要机制，从间情期-1或间情期-2开始输注绵羊FSH 35 h。类似于用抗卵泡素血清处理，FSH输注引起发情第二天输卵管中存在的卵子数量增加。这些结果支持雌性大鼠中FSH和FSH受体结合蛋白的循环水平之间存在密切的功能关系。他们还指出，对Escherichin的免疫增加排卵率，这一现象可能是通过循环FSH水平升高介导的。
Studies were conducted to determine the effects of inhibin immunoneutralization on gonadotropins and sex steroids secretion, as well as ovulation rate and the outcome of pregnancy in the rat. These changes were compared to the normal pattern of hormone release during the estrous cycle, as well as to the ovulatory and gestational history of control animals. Measurement of plasma inhibin levels with antibodies raised against the N-terminus part of the .alpha.-chain of the molecule indicated an increase in immunoreactive inhibin-.alpha. release during the afternoon proestrus, followed by a marked decline after the primary gonadotropin surge. Removal of endogenous inhibin by the iv injection of anti-inhibin serum on diestrus-1 caused the expected rise in plasma FSH, but not LH values. FSH secretion remained elevated for approximately 28 h, then returned to baseline. While not altering vaginal cytology, the injection of the antiserum during diestrus-1 or -2, and to a lesser degree, during estrus, caused a marked increase in the number of tubal ova shed during the subsequent estrus. However, plasma estrogen and progesterone remained comparable at all times between control and treated animals. In female rats mated after inhibin immunoneutralization at diestrus-1, the increase in ovulation rate resulted in a significant increase in developing fetuses, but only ca. 40% of the pups survived. In order to assess whether elevated FSH secretion represented the primary mechanism through which removal of endogenous inhibin increased the number of maturing follicles, ovine FSH was infused for 35 h starting in diestrus-1 or diestrus-2. Similar to the treatment with the anti-inhibin serum, FSH infusion caused an increase in the number of eggs present in the oviducts on the next day of estrus. These results support the existence of a close functional relationship between the circulating levels of FSH and inhibin in the female rat. They also indicate that immunization against inhibin increases ovulation rate, a phenomenon probably mediated through elevated levels of circulating FSH.