Beyond isolated cells: microfluidic transport of large tissue for pancreatic cancer diagnosis.
Beyond isolated cells: microfluidic transport of large tissue for pancreatic cancer diagnosis.
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超越孤立的细胞：用于胰腺癌诊断的大组织的微流体运输。
DOI：
10.1117/12.2076833
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Seibel,EricJ
中科院分区：
文献类型：
--
作者：
Das,Ronnie;Murphy,RachelG;Seibel,EricJ
对于癌症诊断，使用取芯针（CN）从患者获得的芯活检物（CB）传统上由病理学家在样品处理和切片后在显微镜载玻片上可视化和评估。光学信息的基本增益（即，当在3D中分析整个未切片的CB（L = 5-20，D = 0.5-2.0 mm）时，可以实现诊断/分期）。这种方法保留了传统病理学的CB，并最大限度地提高了患者样本的诊断潜力。为了将CN/CB与成像连接起来，我们的小组开发了一种微流体装置，该装置在未切片的CB上进行生物标本制备以用于病理学。最终目标是一个自动化和快速的护理点系统，通过处理先进的3D成像平台的组织来帮助病理学家。一个固有的，但必不可少的设备功能是CB的微流体运输，这是以前没有研究过的。早期实验证明了概念验证：在直/弯曲微通道中输送设定长度的胰腺CB（D = 0.3-2.0 mm），但尺寸公差和流速可变，CB完整性的保持不受控制。第二项研究在微通道中使用金属圆柱替代物（L = 10，D = 1 mm）以理解传输机制。然而，CB是不完美的形状，粗糙，多孔和粘弹性。在本研究中，使用临床CN通过定制界面将新鲜/福尔马林固定的猪和人胰腺CB放置到我们的设备中。CB完整性（即，样品存活率）可以在每个阶段使用光学机械度量进行评估：当样品强度分布数据偏离超过xavg+ 2σ时，确定物理断裂。测定了几种氯化萘的人血细胞流速，并分析了新鲜和福尔马林固定血细胞的微流体转运。
For cancer diagnoses, core biopsies (CBs) obtained from patients using coring needles (CNs) are traditionally visualized and assessed on microscope slides by pathologists after samples are processed and sectioned. A fundamental gain in optical information (i.e., diagnosis/staging) may be achieved when whole, unsectioned CBs (L = 5-20, D = 0.5-2.0 mm) are analyzed in 3D. This approach preserves CBs for traditional pathology and maximizes the diagnostic potential of patient samples. To bridge CNs/CBs with imaging, our group developed a microfluidic device that performs biospecimen preparation on unsectioned CBs for pathology. The ultimate goal is an automated and rapid point-of-care system that aids pathologists by processing tissue for advanced 3D imaging platforms. An inherent, but essential device feature is the microfluidic transport of CBs, which has not been previously investigated. Early experiments demonstrated proof-of-concept: pancreas CBs (D = 0.3-2.0 mm) of set lengths were transported in straight/curved microchannels, but dimensional tolerance and flow rates were variable, and preservation of CB integrity was uncontrolled. A second study used metal cylinder substitutes (L = 10, D = 1 mm) in microchannels to understand the transport mechanism. However, CBs are imperfectly shaped, rough, porous and viscoelastic. In this study, fresh/formalin-fixed porcine and human pancreas CBs were deposited into our device through a custom interface using clinical CNs. CB integrity (i.e., sample viability) may be assessed at every stage using an optomechanical metric: physical breaks were determined when specimen intensity profile data deviated beyond xavg+ 2σ. Flow rates for human CBs were determined for several CNs, and microfluidic transport of fresh and formalin-fixed CBs was analyzed.