Arabidopsis Female Gametophyte Gene Expression Map Reveals Similarities between Plant and Animal Gametes
Arabidopsis Female Gametophyte Gene Expression Map Reveals Similarities between Plant and Animal Gametes
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DOI：
10.1016/j.cub.2010.01.051
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发表时间：
2010-03-23
期刊：
影响因子：
9.2
通讯作者：
Grossniklaus, Ueli
中科院分区：
文献类型：
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作者：
Wuest, Samuel E.;Vijverberg, Kitty;Grossniklaus, Ueli
多细胞生物的发育受到差异基因表达的控制，从而使细胞采取不同的命运。基因表达的空间分辨视图可以阐明与细胞身份和功能相关的转录网络。开花植物的单倍体雌配子体是一种高度简化的生物体：成熟时，它通常由源自共同前体的少至三种细胞类型组成 [1, 2]。然而，由于其难以接近且体积小，我们对雌配子体细胞规范和分化的分子基础知之甚少。在这里，我们报告了成熟拟南芥雌配子体中所有细胞类型的表达谱。差异表达的转录后调节模块和代谢途径表征了不同的细胞类型。与其他植物组织相比，一些转录因子家族在雌配子体中过多，例如 I 型 MADS 结构域、RWP-RK 和生殖分生组织转录因子。 PAZ/Piwi 结构域编码基因在鸡蛋中上调，表明通过小 RNA 途径进行表观遗传调控的作用，这与动物种系中的特征相似 [3]。对人类和拟南芥卵细胞功能组富集的比较发现了一些相似之处，这些相似之处可能代表共同进化或祖先配子特征的结果。
The development of multicellular organisms is controlled by differential gene expression whereby cells adopt distinct fates. A spatially resolved view of gene expression allows the elucidation of transcriptional networks that are linked to cellular identity and function. The haploid female gametophyte of flowering plants is a highly reduced organism: at maturity, it often consists of as few as three cell types derived from a common precursor [1, 2]. However, because of its inaccessibility and small size, we know little about the molecular basis of cell specification and differentiation in the female gametophyte. Here we report expression profiles of all cell types in the mature Arabidopsis female gametophyte. Differentially expressed posttranscriptional regulatory modules and metabolic pathways characterize the distinct cell types. Several transcription factor families are overrepresented in the female gametophyte in comparison to other plant tissues, e.g., type I MADS domain, RWP-RK, and reproductive meristem transcription factors. PAZ/Piwi-domain encoding genes are upregulated in the egg, indicating a role of epigenetic regulation through small RNA pathways a feature paralleled in the germline of animals [3]. A comparison of human and Arabidopsis egg cells for enrichment of functional groups identified several similarities that may represent a consequence of coevolution or ancestral gametic features.