Droplet-based single cell RNAseq tools: a practical guide
Droplet-based single cell RNAseq tools: a practical guide
复制标题
DOI：
10.1039/c8lc01239c
复制
发表时间：
2019-05-21
期刊：
影响因子：
6.1
通讯作者：
Gallego-Ortega, David
中科院分区：
文献类型：
--
作者：
Salomon, Robert;Kaczorowski, Dominik;Gallego-Ortega, David
Drop-Seq、InDrop和Chromium 10X等基于液滴的scRNA-seq系统已成为研究实验室广泛采用高通量scRNA-seq技术的催化剂。为了了解这些系统深入询问生物学的能力；在这里，我们提供了典型scRNA-seq实验中涉及的所有步骤的实用指南。通过比较和对比这三个基于液滴的主要系统(及其衍生物)，我们概述了在获得高质量和生物相关数据方面的所有关键考虑因素。我们还讨论了这些系统的局限性，以及它们如何适应新兴的基因组细胞学领域。
Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.