INVESTIGATIONS INTO THE POSTTRANSLATIONAL MODIFICATION AND MECHANISM OF ISOPENICILLIN N-ACYL-COA ACYLTRANSFERASE USING ELECTROSPRAY MASS-SPECTROMETRY
INVESTIGATIONS INTO THE POSTTRANSLATIONAL MODIFICATION AND MECHANISM OF ISOPENICILLIN N-ACYL-COA ACYLTRANSFERASE USING ELECTROSPRAY MASS-SPECTROMETRY
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DOI：
10.1042/bj2940357
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发表时间：
1993-09-01
影响因子：
4.1
通讯作者：
SCHOFIELD, CJ
中科院分区：
文献类型：
--
作者：
APLIN, RT;BALDWIN, JE;SCHOFIELD, CJ
电喷雾质谱（e.s.m.s.）用于确认异青霉素N：酰基-CoA酰基转移酶前蛋白的翻译后切割的位置，以得到α-和β-亚基。紧急医疗服务。研究表明通过外源加入的取代乙酸在体内对α-亚基进行部分修饰。经前综合征还允许在体外观察酰基从几种酰基辅酶A转移到β亚基。N.m.r. CoA种类的数据已作为补充出版物500173（2页）保藏在大英图书馆文献供应中心（DSC），Boston Spa，Wetherby，West约克郡LS 23 7 BQ，可根据Biochem. J.（1993）289，9中所述的条款从其获得副本。
Electrospray mass spectrometry (e.s.m.s.) was used to confirm the position of the post-translational cleavage of the isopenicillin N: acyl-CoA acyltransferase preprotein to give the alpha- and beta-subunits. The e.s.m.s. studies suggested partial modification of the alpha-subunit in vivo by exogenously added substituted acetic acids. E.s.m.s. has also allowed the observation in vitro of the transfer of the acyl group from several acyl-CoAs to the beta-subunit. N.m.r. data for the CoA species have been deposited as Supplementary Publication SUP 500173 (2 pages) at the British Library Document Supply Centre (DSC), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, from whom copies can be obtained on the terms indicated in Biochem. J. (1993) 289, 9.