Effect of alcohol, acetaldehyde, and salsolinol on beta-endorphin secretion from the hypothalamic neurons in primary cultures.
Effect of alcohol, acetaldehyde, and salsolinol on beta-endorphin secretion from the hypothalamic neurons in primary cultures.
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酒精、乙醛和猪油醇对原代培养物中下丘脑神经元分泌 β-内啡肽的影响。
DOI：
10.1111/j.1530-0277.1993.tb05239.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Sarkar,DK
中科院分区：
文献类型：
--
作者：
Reddy,BV;Sarkar,DK
用原代培养的大鼠胚胎下丘脑神经元研究乙醇、乙醛和猪毛菜醇对下丘脑β-内啡肽分泌神经元的影响。将这些神经元细胞暴露于不同浓度的乙醇（12.5-50 mM）和乙醛（12.5 - 50 μm），导致β-内啡肽分泌的浓度依赖性增加。猪毛菜醇（12.5-50 μm）不会引起β-内啡肽分泌的任何显著变化。乙醇的作用是短暂的（2小时）。与乙醇相比，乙醛对β-内啡肽分泌的影响更大，持续时间更长。乙醇和猪毛菜醇对细胞活力没有任何影响，而较高浓度的乙醛似乎在处理6小时后减少活细胞的数量。上述处理均对细胞DNA含量无任何影响。这些结果表明，乙醇是下丘脑β-内啡肽的有效刺激剂。这些结果还首次表明，乙醇的代谢物乙醛在刺激β-内啡肽分泌方面更有效，并且在乙醇调节的β-内啡肽分泌中可能是重要的。
The effect of ethanol, acetaldehyde, and salsolinol on hypothalamicβ‐endorphin secreting neurons is studied by using rat fetal hypothalamic neurons in primary culture. Exposure of these neuronal cells to different concentrations of ethanol (12.5–50 mM) and acetaldehyde (12.5‐50 μm) caused a concentration‐dependent increase in the secretion ofβ‐endorphin. Salsolinol (12.5–50 μm) did not cause any significant change in the secretion ofβ‐endorphin. Ethanol's effect was short‐lasting (2 hr). Acetaldehyde's effect onβ‐endorphin secretion was greater and longer lasting, as compared with ethanol. Ethanol and salsolinol do not have any effect on cell viability, whereas higher concentrations of acetaldehyde appear to reduce the number of viable cells after 6 hr of treatment. None of the above treatments has any effect on cellular DNA content. These results suggest that ethanol is a potent stimulator of hypothalamicβ‐endorphin. These results also show for the first time that ethanol's metabolite acetaldehyde is more potent in stimulatingβ‐endorphin secretion and may be significant in the ethanol regulatedβ‐endorphin secretion.