O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates.
O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates.
复制标题
灿烂弧菌分离株中 wbe 区域的 O 抗原多样性和横向转移。
DOI：
10.1111/j.1462-2920.2010.02274.x
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发表时间：
2010
影响因子：
5.1
通讯作者：
Polz,MartinF
中科院分区：
文献类型：
--
作者：
Wildschutte,Hans;Preheim,SarahP;Hernandez,Yasiel;Polz,MartinF
O抗原是在革兰氏阴性菌的外表面上表达的高度多样化的结构。负责O抗原合成的产物在wbe区域编码，该区域具有广泛的遗传多样性。虽然在弧菌种内观察到异质性O抗原，但这些结构的表征几乎专门用于病原体。在这里，我们调查了沿海marineVibrio splendidus-like分离株之间的O抗原多样性。首先使用菌株LGP 32、12 B 01和Med 222的测序基因组鉴定和表征了wberegion。这些区域具有遗传多样性，反映了其表达的O抗原。基于多位点序列分析（MLSA）和O抗原谱，进一步表征了来自Plum Island Estuary（MA，USA）物理上不同栖息地的其他分离株，包括动物宿主内和悬浮颗粒上的分离株。结果表明，在生态环境中的血清型多样性。在48个MLSA基因完全相同的菌株中，41个菌株表现出gpm遗传多样性，这是一个与该菌株紧密连锁的基因，至少有12个菌株表达不同的O抗原谱，进一步证明了该菌株的遗传多样性。我们的研究结果表明，在这些环境菌株中O抗原高度变异，并表明频繁的横向基因转移产生了广泛的多样性。和它的近亲。
The O‐antigen is a highly diverse structure expressed on the outer surface of Gram‐negative bacteria. The products responsible for O‐antigen synthesis are encoded in thewberegion, which exhibits extensive genetic diversity. While heterogeneous O‐antigens are observed withinVibriospecies, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O‐antigen diversity among coastal marineVibrio splendidus‐like isolates. Thewberegion was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O‐antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O‐antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showedgpmgenetic diversity, a gene closely linked to thewbelocus, and at least 12 expressed different O‐antigen profiles further suggestingwbegenetic diversity. Our results demonstrate O‐antigen hyper‐variability among these environmental strains and suggest that frequent lateral gene transfer generateswbeextensive diversity amongV. splendidusand its close relatives.