Oxidative Capacity of Nanobubbles and Its Effect on Seed Germination
Oxidative Capacity of Nanobubbles and Its Effect on Seed Germination
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DOI：
10.1021/acssuschemeng.5b01368
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发表时间：
2016-03-01
影响因子：
8.4
通讯作者：
Uchida, Tsutomu
中科院分区：
文献类型：
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作者：
Liu, Shu;Oshita, Seiichi;Uchida, Tsutomu
据报道，纳米气泡(NBS)可以有效地加速生物的新陈代谢，但其机制尚未被很好地了解。本文研究了NBS产生的活性氧(ROS)及其对种子萌发的影响。测定了APF对Nb水的荧光响应。它随Nb数密度的变化而变化，Nb数密度随储存时间的延长而减小。这说明NBS能产生ROS，且ROS的量与Nb数密度呈正相关。在0-1 mM范围内，APF的荧光强度与H_2O_2浓度呈线性关系。在Nb产生后不久，氧气Nb水和气体混合物(空气+氮气)Nb水的ROS量分别相当于0.5 mM和0.3 mM H_2O_2。种子在NB水、蒸馏水和过氧化氢溶液中进行萌发试验。用Nb水和0.3 mM H_2O_2混合气体浸种的种子各观察时间的发芽率均高于蒸馏水浸种。用NBT染色检测种子中超氧阴离子自由基的含量。吸光度数据的结果表明，在混合气体NB水和0.3 mM H_2O_2溶液中浸泡的种子中的O-2(中心点-)量相似，且显著高于蒸馏水中的O-2(中心点-)。这些结果表明，NB水产生的中等水平的外源ROS对种子萌发起重要作用。
Nanobubbles (NBs) have been reported to be effective at accelerating the metabolism of living organisms, but the mechanism is not yet well understood. In this study, the production of reactive oxygen species (ROS) by NBs and its effect on seed germinations were investigated. The fluorescence response of APF to NB water was measured. It changed depending on the NB number density which decreased with storage time. This indicated that NBs could produce ROS and the amount of ROS had positive correlation with the NB number density. The fluorescence intensity of APF increases linearly with the concentration of H2O2 in the range of 0-1 mM. Just after the NB generation, the oxidative capacities represented by amount of ROS of oxygen NB water and gas-mixture (air + nitrogen) NB water were estimated to be equivalent to 0.5 and 0.3 mM H2O2 respectively. The seed germination tests were performed in the NB water, distilled water and H2O2 solutions. The germination rate at each observation times of seeds submerged in gas-mixture NB water and 0.3 mM H2O2 solutions were both higher than those submerged in distilled water. The amounts of superoxide radicals in the seeds were detected using NBT staining. The results of absorbance data proved that the amounts of O-2(center dot-) in seeds submerged in gas-mixture NB water and in 0.3 mM H2O2 solution were similar and significantly higher than those in the distilled water. These results indicated that moderate level of exogenous ROS produced by NB water played an important role in seed germination.