Association of human polyomavirus JC with peripheral blood of immunoimpaired and healthy, individuals
Association of human polyomavirus JC with peripheral blood of immunoimpaired and healthy, individuals
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DOI：
10.1080/13550280390195379
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发表时间：
2003-01-01
影响因子：
3.2
通讯作者：
Dörries, R
中科院分区：
文献类型：
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作者：
Dörries, K;Sbiera, S;Dörries, R
JC病毒（JCV）感染在健康个体中通常无症状。相反，在免疫功能低下的个体中，高度活化的病毒复制可能导致PML。在健康和患病个体中发现外周血细胞（PBMC）含有JCV DNA，并且讨论了它们可能负责病毒在持久性期间传播到中枢神经系统（CNS）。为了更好地了解JCV DNA在PBMC中持续感染和发病机制中的作用，作者描述了健康和人类免疫缺陷病毒1型（HIV-1）感染个体的Ficoll梯度纯化血细胞（外周血单核细胞[PBMC]）中JCV感染的程度。在低敏感性聚合酶链反应（PCR）中，发现健康JCV感染者PBMC中的病毒活化率为0%至38%。在进行性多灶性白质脑病（PML）患者中，发现了更强的信号，表明病毒活化增加。在T、B淋巴细胞和单核细胞中检测到低水平的JCV DNA。然而，粒细胞被证明是JCV DNA的主要储存库，具有高拷贝数。虽然病毒基因组的总体分布适用于所研究的人群，但在个体中，可以发现分布模式发生了显着变化。
JC virus (JCV) infection is regularly asymptomatic in healthy individuals. In contrast, in immunocompromised individuals, highly activated virus replication may lead to PML. Peripheral blood cells (PBCs) are found to harbor JCV DNA in healthy and diseased individuals and it is discussed that they might be responsible for dissemination of the virus to the central nervous system (CNS) during persistence. To better understand the role of JCV DNA in PBCs for persistent infection and pathogenesis, the authors characterized the extent of JCV infection in Ficoll-gradient purified blood cells (peripheral blood mononuclear cells [PBMCs]) of healthy and human immunodeficiency virus type 1 (HIV-1)-infected individuals. Virus activation in PBMCs from healthy JCV-infected individuals was found at a rate of 0% to 38% at low polymerase chain reaction (PCR) sensitivity. In progressive multifocal leukoencephalopathy (PML) patients, a stronger signal was found, indicating increased virus activation. JCV DNA was regularly detected in T and B lymphocytes and in monocytes at low levels. However, granulocytes were shown to be the predominant reservoir of JCV DNA harboring high copy numbers. Although the overall distribution of viral genomes holds true for the population studied, in the individual, a markedly changed pattern of distribution can be found.