Low-temperature tolerance of the Antarctic species Deschampsia antarctica: A complex metabolic response associated with nutrient remobilization
Low-temperature tolerance of the Antarctic species Deschampsia antarctica: A complex metabolic response associated with nutrient remobilization
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DOI：
10.1111/pce.13737
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发表时间：
2020-02-24
影响因子：
7.3
通讯作者：
Gago, Jorge
中科院分区：
文献类型：
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作者：
Clemente-Moreno, Maria Jose;Omranian, Nooshin;Gago, Jorge
Deschampsia anapartica（DA）是南极洲仅有的两种原生维管物种之一。我们进行了生理生态学，生物化学和代谢组学研究，以探讨DA对低温的反应。同时，我们评估了来自同一家族（禾本科）的非南极参考物种（小麦[TA]）的反应。在低温（4摄氏度），这两个物种表现出较低的光合速率（减少70%和80%的DA和TA，分别）和氧化应激的症状，但相反的抗氧化酶（过氧化物酶和过氧化氢酶）的反应。我们采用融合的最小绝对收缩和选择算子统计建模相关联的物种依赖的生理和抗氧化反应的初级代谢。DA的模型结果表明，与植物保护，细胞壁重塑，膜稳定，和抗氧化剂的次生代谢（黄酮醇和苯丙素类化合物的合成），协调与营养动员从源到库组织（元素分析证实），这是没有观察到TA。DA的代谢行为，特别是代谢物的显着变化，进行了比较与一个新编译的多物种数据集显示一般的代谢物在低温下的积累。总而言之，DA显示的反应表明分解代谢和叶功能维持之间的妥协。
The species Deschampsia antarctica (DA) is one of the only two native vascular species that live in Antarctica. We performed ecophysiological, biochemical, and metabolomic studies to investigate the responses of DA to low temperature. In parallel, we assessed the responses in a non-Antarctic reference species (Triticum aestivum [TA]) from the same family (Poaceae). At low temperature (4 degrees C), both species showed lower photosynthetic rates (reductions were 70% and 80% for DA and TA, respectively) and symptoms of oxidative stress but opposite responses of antioxidant enzymes (peroxidases and catalase). We employed fused least absolute shrinkage and selection operator statistical modelling to associate the species-dependent physiological and antioxidant responses to primary metabolism. Model results for DA indicated associations with osmoprotection, cell wall remodelling, membrane stabilization, and antioxidant secondary metabolism (synthesis of flavonols and phenylpropanoids), coordinated with nutrient mobilization from source to sink tissues (confirmed by elemental analysis), which were not observed in TA. The metabolic behaviour of DA, with significant changes in particular metabolites, was compared with a newly compiled multispecies dataset showing a general accumulation of metabolites in response to low temperatures. Altogether, the responses displayed by DA suggest a compromise between catabolism and maintenance of leaf functionality.