A subpopulation of gonadotropin-releasing hormone neurons in the adult mouse forebrain is gamma-Aminobutyric acidergic.
A subpopulation of gonadotropin-releasing hormone neurons in the adult mouse forebrain is gamma-Aminobutyric acidergic.
复制标题
成年小鼠前脑中促性腺激素释放激素神经元的亚群具有γ-氨基丁酸能。
DOI：
10.1002/jnr.23610
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Xiang Zhenghua
中科院分区：
文献类型：
--
作者：
Zhu Jiao;Xu Xiao-hui;Knight Gillian E;He Cheng;Burnstock Geoffrey;Xiang Zhenghua
促性腺激素释放激素（GnRH）神经元在生殖功能中发挥着关键作用。 GnRH 以受神经递质或神经调节剂调节的不同脉冲形式释放。通过免疫组织化学和 GAD67-GFP 敲入小鼠，这项研究首次表明成年小鼠前脑中的 GnRH 神经元子集具有 γ-氨基丁酸 (GABA) 能。雌性小鼠与雄性小鼠中表达 GAD67-GFP 的 GnRH 神经元的百分比存在性别差异。雌性小鼠去势后表达 GAD67-GFP 的 GnRH 神经元百分比下降，雌二醇治疗后增加至正常雌性水平。在完整、去势或去势+丙酸睾酮治疗的雄性小鼠中，表达 GAD67-GFP 的 GnRH 神经元的百分比没有显着变化。在雌性发情周期中，发情期表达 GAD67-GFP 的 GnRH 神经元的百分比高于动情期。在出生后发育的性成熟过程中，GnRH神经元直到出生后第15天（P）才表达GAD67-GFP，并且在P30（对应于成熟阶段）时首次检测到性别差异。总之，我们的数据表明：1）小鼠前脑中的 GnRH 神经元子集具有 GABA 能，2）GnRH 神经元中 GAD67-GFP 的表达至少部分受雌激素调节，3）GnRH 神经元分泌 GABA 来调节自身。
Gonadotropin-releasing hormone (GnRH) neurons play a pivotal role in reproductive function. GnRH is released in distinct pulses that are regulated by neurotransmitters or neuromodulators. With immunohistochemistry and GAD67-GFP knockin mice, this study shows for the first time that a subset of GnRH neurons in the forebrain of adult mouse is γ-aminobutyric acid (GABA)-ergic. There is a gender difference in the percentage of GnRH neurons expressing GAD67-GFP in female vs. male mice. The percentage of GnRH neurons expressing GAD67-GFP decreased after castration of female mice and increased to the normal female level after estradiol treatment. The percentage of GnRH neurons expressing GAD67-GFP did not change significantly in intact, castrated, or castration + testosterone propionate-treated male mice. During the female estrous cycle, the percentage of GnRH neurons expressing GAD67-GFP was higher during the estrous stage than during the diestrous stage. During sexual maturation of postnatal development, GnRH neurons did not express GAD67-GFP until postnatal day (P) 15, and the gender differences were first detected at P30, which corresponds to the maturation stage. In conclusion, our data suggest that 1) a subset of GnRH neurons in mouse forebrain is GABA-ergic, 2) expression of GAD67-GFP in GnRH neurons is at least in part regulated by estrogen, and 3) GnRH neurons secrete GABA to regulate themselves.