Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein
Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein
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DOI：
10.1073/pnas.96.18.10284
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发表时间：
1999-08-31
影响因子：
11.1
通讯作者：
Yoshimura, A
中科院分区：
文献类型：
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作者：
Ashikari, M;Wu, JZ;Yoshimura, A
利用定位克隆技术鉴定了水稻矮秆1基因。它的隐性突变等位基因赋予侏儒表型。连锁分析显示，编码gtp结合蛋白cr亚基的cDNA在3185个dl片段中与dl共分离。以该cDNA为探针的Southern杂交分析显示，在不同的dl突变系中存在不同的条带模式。在至少4个独立的dl突变体中，Northern杂交分析未观察到基因转录，测序分析显示其中一个突变等位基因缺失833 bp，导致无法表达gtp结合蛋白，一个带有gtp结合蛋白基因的转基因dl突变体恢复了正常表型。我们认为，水稻矮1号基因编码gtp结合蛋白，该蛋白在植物生长发育中起重要作用。由于dl突变体被归类为赤霉素不敏感，我们认为gtp结合蛋白可能与赤霉素信号转导有关。
A rice Dwarf 1 gene was identified by using a map-based cloning strategy. Its recessive mutant allele confers a dwarf phenotype. Linkage analysis revealed that a cDNA encoding the cr-subunit of GTP-binding protein cosegregated with dl in 3,185 dl segregants. Southern hybridization analysis with this cDNA as a probe showed different band patterns in several dl mutant lines. In at least four independent dl mutants, no gene transcript was observed by Northern hybridization analysis, Sequencing analysis revealed that an 833-bp deletion had occurred in one of the mutant alleles, which resulted in an inability to express GTP-binding protein, A transgenic dl mutant with GTP-binding protein gene restored the normal phenotype. We conclude that the rice Dwarf I gene encodes GTP-binding protein and that the protein plays an important role in plant growth and development. Because the dl mutant is classified as gibberellin-insensitive, we suggest that the GTP-binding protein might be associated with gibberellin signal transduction.