Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativa L.) by affecting oxalate exudation from root tips
Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativa L.) by affecting oxalate exudation from root tips
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脱水蛋白 MsDHN1 通过影响根尖的草酸盐渗出来提高苜蓿 (Medicago sativa L.) 的铝耐受性
DOI：
10.1111/tpj.15451
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发表时间：
2021-08-25
期刊：
影响因子：
7.2
通讯作者：
An, Yuan
中科院分区：
文献类型：
--
作者：
Lv, Aimin;Wen, Wuwu;An, Yuan
从紫花苜蓿（Medicagosativa L.）中克隆了一个SK 3型转基因植物MsDHN 1。通过在苜蓿幼苗或毛状根中过表达和抑制MsDHN 1来研究其功能和基因调控途径。结果表明，MsDHN 1是一种典型的内在无序蛋白，在苜蓿中以单体和同源二聚体的形式存在。在铝胁迫下，与野生型或载体相比，苜蓿幼苗或毛状根中MsDHN 1过表达（MsDHN 1-OE）的植株生长速率增加，MsDHN 1-RNAi抑制（MsDHN 1-RNAi）的植株生长速率降低。MsDHN 1与水通道蛋白（AQP）、MsPIP 2;1和MsTIP 1;1相互作用，促进根尖草酸的分泌和铝的积累。MsABF 2是MsDHN 1的上游转录因子，通过与MsDHN 1启动子的ABRE元件结合而激活MsDHN 1的表达。MsABF 2对MsDHN 1的转录调控依赖于脱落酸信号通路。以上结果表明，MsDHN 1通过增加苜蓿根尖草酸的分泌来提高苜蓿对铝胁迫的耐受性，这可能与MsDHN 1与两个水通道蛋白的相互作用有关。
A SK3-type dehydrin MsDHN1 was cloned from alfalfa (Medicago sativa L.). Its function and gene regulatory pathways were studied via overexpression and suppression of MsDHN1 in alfalfa seedlings or hairy roots. The results showed that MsDHN1 is a typical intrinsically disordered protein that exists in the form of monomers and homodimers in alfalfa. The plant growth rates increased as a result of MsDHN1 overexpression (MsDHN1-OE) and decreased upon MsDHN1 suppression (MsDHN1-RNAi) in seedlings or hairy roots of alfalfa compared with the wild-type or the vector line under Al stress. MsDHN1 interacting with aquaporin (AQP) MsPIP2;1 and MsTIP1;1 positively affected oxalate secretion from root tips and Al accumulation in root tips. MsABF2 was proven to be an upstream transcription factor of MsDHN1 and activated MsDHN1 expression by binding to the ABRE element of the MsDHN1 promoter. The transcriptional regulation of MsABF2 on MsDHN1 was dependent on the abscisic acid signaling pathway. These results indicate that MsDHN1 can increase alfalfa tolerance to Al stress via increasing oxalate secretion from root tips, which may involve in the interaction of MsDHN1 with two AQP.