The 5′-flanking regions of CYP19A1 and CYP19A2 in zebrafish
The 5′-flanking regions of CYP19A1 and CYP19A2 in zebrafish
复制标题
DOI：
10.1006/bbrc.2001.5796
复制
发表时间：
2001-11-02
影响因子：
3.1
通讯作者：
Trant, JM
中科院分区：
文献类型：
--
作者：
Kazeto, YK;Ijiri, S;Trant, JM
本报告描述了从斑马鱼（Danio rerio）基因组中分离的CYP 19 A1和A2基因的5 '侧翼区的结构。三个cAMP反应元件（CRE）的共有序列，一个芳香烃反应元件在CYP 19 A1的5 '侧翼区观察到一个类固醇生成因子I（SF-1）位点（AhR/Arnt）和一个TATA盒。相反，CYP 19 A2的5'侧翼区位于一个非翻译外显子的上游，具有一个CRE、一个雌激素反应元件（ERE）、过氧化物酶体增殖物激活受体α/类维生素A X受体α异二聚体反应元件（PPAR α/RXR α）和TATA盒。引物延伸分析显示，CYP 19 A1和A2转录本的主要转录起始位点分别位于推定的翻译起始密码子上游28和91 bp处。这些分析表明，实质上不同的监管机构，包括各种环境异生物质，控制两个CYP 19基因的表达。(C)北京：科学出版社.
This report describes the structure of the 5'-flanking regions of both the CYP19A1 and A2 genes that were isolated from the genome of the zebrafish (Danio rerio). Consensus sequences of three cAMP-responsive elements (CRE), an aryl hydrocarbon-responsive element (AhR/Arnt), a steroidogenic factor I (SF-1) site, and a TATA box were observed in the 5'-flanking region of CYP19A1 In contrast, the 5'-flanking region of CYP19A2 was located upstream of an untranslated exon and possessed consensus sequences of a single CRE, an estrogen-responsive element (ERE), a peroxisome proliferator-activated receptor alpha /retinoid X receptor alpha heterodimer-responsive element (PPAR alpha /RXR alpha), and a TATA box. Primer extension analysis revealed that the predominant transcription initiation sites for CYP19A1 and A2 transcripts were 28 and 91 bp upstream from the putative translation initiation codon, respectively. These analyses indicate that substantially different regulators, including a variety of environmental xenobiotics, control the expression the two CYP19 genes. (C) 2001 Academic Press.