The taxonomic position of Asian Holopedium (Crustacea: Cladocera) confirmed by morphological and genetic analyses
The taxonomic position of Asian Holopedium (Crustacea: Cladocera) confirmed by morphological and genetic analyses
复制标题
DOI：
10.1007/s10201-019-00585-z
复制
发表时间：
2019-07
期刊：
影响因子：
1.6
通讯作者：
Ayaka Yamamoto;W. Makino;J. Urabe
中科院分区：
文献类型：
--
作者：
Ayaka Yamamoto;W. Makino;J. Urabe
cladoceranHolopedium gibberumZaddach, 1855（栉足目：holopeidae）曾被认为广泛分布于北半球，但其隐生的姐妹种最近才从h。文章标题严格赤霉素（Holopedium glacialis）一新种。在东亚，尽管“H。赤霉素在许多水体中都有记录，但调查种群的身份很少通过分子分析得到证实。因此，尚不清楚它是否真的是h。gibberums.s。orH。分布在东亚的冰川作用。我们利用dna条形码技术对日本采得的荷兰花标本进行分类鉴定。我们对日本holopediumd线粒体细胞色素氧化酶亚基1 （mtCOI）和核18S核糖体DNA （nr18S）进行了测序，并与日本holopediumd的结果进行了比较。gibberums.s。收集于挪威和h。在加拿大收集的冰川资料。挪威页岩的mtCOI序列差异。gibberums.s。和日本holopedium最多为2.4%，在进化纲中属于种内分化程度。NorwegianH。gibberums.s。和日本龙葵共有相同的nr18S单倍型。加拿大h。冰川期与日本全影属的冰川期基因不同。因此，我们得出结论，日本holopedium可以识别asH。gibberums.s。
The cladoceranHolopedium gibberumZaddach, 1855 (Ctenopoda: Holopediidae) was once thought to occur broadly in the northern hemisphere, but its cryptic sister species was recently separated fromH. gibberumsensu stricto (s.s.) as a new species,Holopedium glacialis. In East Asia, although “H. gibberum” occurrence has been recorded in many water bodies, the identity of the surveyed populations has rarely been confirmed via molecular analyses. Thus, it is unclear whether it is actuallyH. gibberums.s. orH. glacialisthat is distributed in East Asia. We used DNA-barcoding techniques to check the taxonomic status ofHolopediumsamples collected in Japan. We sequenced mitochondrial cytochromecoxidase subunit 1 (mtCOI) and nuclear 18S ribosomal DNA (nr18S) of JapaneseHolopediumand compared the results with those ofH. gibberums.s. collected in Norway andH. glacialiscollected in Canada. The mtCOI sequence divergences between NorwegianH. gibberums.s. and JapaneseHolopediumwere at most 2.4%, which was within the degree of intraspecific differentiation in cladocerans. NorwegianH. gibberums.s. and JapaneseHolopediumshared identical nr18S haplotypes. Individuals of CanadianH. glacialiswere genetically different from those of JapaneseHolopedium. We therefore concluded that JapaneseHolopediumcan be identified asH. gibberums.s.