On pathways for small molecules into and out of human hair fibers.
On pathways for small molecules into and out of human hair fibers.
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关于小分子进出人类头发纤维的途径。
DOI：
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发表时间：
1996
影响因子：
1.6
通讯作者：
M. Moeller
中科院分区：
文献类型：
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作者：
L. Pötsch;M. Moeller
本文介绍了人类头发纤维染色和扩散现象的组织学实验方法及其对解释药物滥用头发分析结果的贡献。将罗丹明B从水溶液或甲醇/乙醇溶剂中应用于人发纤维。这些实验是在不同种族群体的天然头发以及广泛漂白的发束上进行的。对罗丹明B扩散到毛发纤维中的途径的显微镜研究表明，该试剂已在角质层细胞之间的鳞片边缘处进入未改性的纤维。在扩散过程开始时，细胞间扩散是主要沿着细胞膜复合物（CMC）和巨纤维间粘固剂的非角质化区域的优选途径。当在水溶液中进行染色时，渗透到纤维的高硫区域中，并导致均匀染色的横截面。在自然头发暴露于非水溶液中观察到的染料分布模式表明，罗丹明B不渗透皮层细胞容易从水溶液和选择性染色nonkeratinous区域。通过分光光度法测定纤维吸收的染料量表明，样品扩散一般随时间和温度而增加。这也取决于头发样本的形态。罗丹明B在水溶液中的渗透性远大于在甲醇/乙醇溶剂中的渗透性。
This paper represents an experimental approach of histology of the human hair fiber in dyeing and diffusion phenomena and its contribution to the interpretation of hair analysis results for drug abuse. Rhodamine B was applied to human hair fibers from either aqueous solution or methanol/ethanol solvent. The experiments were performed on natural hair of different ethnic groups as well as on extensively bleached hair strands. The microscopical study of the pathway of diffusion of rhodamine B into the hair fibers indicated that the reagent had entered the unmodified fibers at the scale edges between the cuticle cells. At the beginning of the diffusion process intercellular diffusion was the preferred route predominantly along the nonkeratinous regions of the cell membrane complex (CMC) and intermacrofibrillar cement. Penetration into the high sulfur regions of the fiber occurred as dyeing in aqueous solution proceeded and resulted in evenly stained cross sections. The dye distribution pattern observed in natural hair exposed to nonaqueous solution showed that rhodamine B did not penetrate the cortex cells as easy as from aqueous solution and selectively stained nonkeratinous regions only. The determination of the amount of dye taken up by the fibers by spectrophotometric analysis demonstrated that samples diffusion generally increased by time and temperature. It also depended on the morphology of the hair sample. The penetration of rhodamine B from aqueous solution was much greater than from methanol/ethanol solvent.