ERYTHROID TRANSCRIPTION FACTOR GATA-1 IS ABUNDANTLY TRANSCRIBED IN MOUSE TESTIS
ERYTHROID TRANSCRIPTION FACTOR GATA-1 IS ABUNDANTLY TRANSCRIBED IN MOUSE TESTIS
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DOI：
10.1038/362466a0
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发表时间：
1993-04-01
期刊：
影响因子：
64.8
通讯作者：
YAMAMOTO, M
中科院分区：
文献类型：
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作者：
ITO, E;TOKI, T;YAMAMOTO, M
转录因子GATA-1是造血细胞系1，2中基因的基本调节因子，属于与共有序列WGATAR3-5结合的因子家族。GATA基序最初是在珠蛋白和其他红系特异基因的顺式调节区6，7中发现的，但后来由GATA因子控制的基因范围扩大到1，7-10。GATA转录因子家族的成员共享一个保守的锌指DNA结合域，但每个GATA因子的表达谱都是不同的11-13。在这里，我们证明了睾丸形式的小鼠(M)GATA-1信使RNA从位于5‘的启动子转录到红系第一外显子，其余外显子(编码mGATA-1蛋白)被睾丸和红系转录物共同使用。我们用抗mGATA-1的单抗表明，在红系细胞中表达的因子与在小鼠睾丸生精小管中发现的相同。在10周龄的睾丸中，GATA-1的表达细胞仅与生精小管的基底膜接触，这表明GATA-1在精子发生的早期阶段对基因进行调控。
THE transcription factor GATA-1 is a fundamental regulator of genes in haematopoietic cell lineages1,2 and belongs to a family of factors that bind to the consensus sequence WGATAR3-5. The GATA motif was originally identified in cis-regulatory regions of globin and other erythroid-specific genes6,7, but the range of genes controlled by the GATA factors has since expanded1,7-10. Members of the GATA transcription factor family share a conserved zinc-finger DNA-binding domain, but the expression profile of each GATA factor is distinct11-13 . Here we show that a testis form of murine (m)GATA-1 messenger RNA is transcribed from a promoter located 5' to the erythroid first exon, and the remaining exons (which encode the mGATA-1 protein) are used in common by both testis and erythroid transcripts. We use an anti-mGATA-1 monoclonal antibody to show that the factor expressed in erythroid cells is the same as that found in the seminiferous tubules of murine testis. The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis.