Promoter analysis and transcription regulation of fus gene cluster responsible for fusaricidin synthesis of Paenibacillus polymyxa SQR-21
Promoter analysis and transcription regulation of fus gene cluster responsible for fusaricidin synthesis of Paenibacillus polymyxa SQR-21
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多粘类芽孢杆菌SQR-21杀镰刀菌素合成fus基因簇的启动子分析及转录调控
DOI：
10.1007/s00253-013-5157-6
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发表时间：
2013-11-01
影响因子：
5
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
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作者：
Li, Shuqing;Zhang, Ruifu;Shen, Qirong
多粘类芽孢杆菌产生的杀镰孢菌素是一种脂肽类抗生素，具有显著的抗真菌活性。本研究从多粘青霉SQR-21（P. polymyxa SQR-21）的cDNA文库中分离并鉴定了杀镰孢菌素生物合成基因（fusA）。MALDI-TOF MS分析证实SQR-21能产生A、B、C和D四种杀镰孢菌素。通过对fus启动子区域进行5′端缺失定位，发现了一个驱动fusGFEDCBA转录的中心启动子。SQR-21中fusA的破坏导致杀镰孢菌素的产生和抗真菌活性的消除。电泳迁移率变动分析证实了一个潜在的调节因子AbrB与fus基因簇的启动子区域之间的直接相互作用。一个abrB破坏突变体表现出显着更高的抗真菌活性与野生型相比。这些结果揭示了多粘原杆菌fus基因簇的转录调控途径。
Fusaricidins produced by Paenibacillus polymyxa are lipopeptide antibiotics with outstanding antifungal activity. In this study, the whole gene cluster responsible for fusaricidin biosynthesis (fusA) was isolated and identified from the cDNA library of one biocontrol agent P. polymyxa SQR-21 (SQR-21). MALDI-TOF MS analysis confirmed that SQR-21 could produce four kinds of fusaricidins: A, B, C, and D. A central promoter that drove the transcription of fusGFEDCBA was revealed by mapping of the fus promoter region by 5′ deletions. The disruption of fusA in SQR-21 led to the abolishment of fusaricidin production and antifungal activity. The direct interaction between a potential regulator, AbrB, and the promoter region of fus gene cluster was confirmed by electrophoretic mobility shift assays. One abrB disruption mutant showed significantly higher antifungal activity compared with the wild type. These results revealed a pathway for the transcriptional regulation of the fus gene cluster in P. polymyxa.